The Impact of Reduced Bisphosphoglycerate Mutase(BPGM)Enzyme Activity in Sensitized Red Blood Cells on Oxygen Carrying/Releasing Capacity
Objective To explore the underlying causes for the observed anomalies,the oxygen-carrying/releasing capacity of sensitized red blood cells(RBCs)was analyzed.Methods The sensitized red blood cells were detected through microcolumn gel cards,and an oxygen-carrying/releasing assessment device was employed to evaluate the oxygen-carrying/releasing capacity of sensitized RBCs in patients with autoimmune hemolytic anemia(AIHA)and hemolytic disease of the newborn(HDN).We constructed a transfusion-induced hemolytic reaction mouse model using plasma from healthy adults,while the control group received PBS.Record the time until exhaustion during weight-loaded swimming in mice and analyze the oxygen-carrying/releasing capacity of isolated mouse RBCs.Perform GO and KEGG enrichment analysis on differentially expressed proteins related to hemolysis and the control group using proteomics.Measure BPGM enzyme activity and 2,3-DPG levels in sensitized RBCs using ELISA.Results Compared to the corresponding control groups,the oxygen dissociation curves in AIHA,HDN,and transfusion-induced hemolytic reaction model mice exhibited a left shift and decreased P50.The weight-loaded swimming time in hemolytic model mice was significantly shortened.Proteomic screening revealed abnormalities in BPGM,with decreased BPGM enzyme activity and a decline in intracellular 2,3-DPG content.Conclusion The reduced activity of BPGM in sensitized RBCs led to a decline in their oxygen-releasing capacity.
万方数据
维普
