Objective This study aims to analyze the efficiency,advantages and mechanisms of the ONT platform,a TGS method in ambiguous results of the HLA-DPB1 genotyping in comparison to PCR-SBT and NGS.Methods 48 samples were tested for HLA-DPB1 genotyping by PCR-SBT,NGS(Illumina platform)and TGS(ONT platform).HLA-DPB1 allele was assigned to the third field for NGS and TGS,and the second field for PCR-SBT.Results TGS had no ambiguity with a single allele combination of HLA-DPB1 alleles.Ambiguous results were given in 45 out of 48 samples by PCR-SBT,accounting for 93％(45/48),and 5 out of 48 samples by NGS,accounting for 10％(5/48).Conclusion Compared with NGS and PCR-SBT,TGS can resolve most of the ambiguous combinations and improve the efficiency and accuracy in HLA-DPB1.
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