Effect of 4 ℃ Cold-Stored Platelets Transfusion on Acute Renal Injury Induced by Hemorrhagic Shock in Rats
Objective To investigate the effects of 4 ℃ cold-stored platelets(cold storage platelets,CSP)transfusion on acute kidney injury caused by hemorrhagic shock/resuscitation(HS/R)in rats.Methods 20 healthy male SD rats were randomly divided into 4 groups:sham operation group(blank group),HS/R model group(model group),CSP transfusion group,room temperature-stored platelets(RTP)kept at 22℃transfusion group(RTP transfusion group).The rats were executed 24 hours after volume resuscitation,and blood and kidney tissue specimens were collected.Serum concentrations of creatinine(Scr),urea nitrogen(BUN),cystatin C(CysC)and interleukin-1β(IL-1β)and interleukin-18(IL-18)in renal tissue were detected in each group.The expression levels of neutrophil gelatinase-associated lipid carrier protein(NGAL)and renal injury cause-1(KIM-1)in renal tissue were detected by Western Blot method,and the pathological changes of renal tissue were observed by HE staining.Results ①There were no significant differences in baseline body mass,MAP,SCr,BUN and other indexs among four groups(P＞0.05).②24 h after resuscitation,the levels of SCr(P＜0.05),BUN(P＜0.01),CysC(P＜0.01)were higher in the model group than in the other three groups;the levels of BUN(P＜0.01),SCr(P=0.037),CysC(P=0.020)in the CSP infusion group were lower than those in the RTP infusion group.③Compared with the model group,serum IL-1β and IL-18 levels were significantly lower in both CSP infusion group and RTP infusion group.Compared with the RTP infusion group,serum IL-1β levels were reduced in the CSP infusion group(P=0.041).Compared with model group,NGAL protein expression was significantly decreased in CSP infusion group(P＜0.01),and KIM-1 protein expression was significantly decreased in both CSP infusion group and RTP infusion group(P＜0.01).Renal histopathological results showed that CSP transfusion could reduce the degree of renal cell degeneration,necrosis and inflammatory cell infiltration.Conclusion Compared with RTP,CSP transfusion could reduce HS/R acute kidney injury in SD rats.
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