Biological Effects of Human Serum Albumin from Different Sources on Vascular Endothelial Cells
Objective To evaluate the differential biological effects of human serum albumin(HSA)derived from different sources on vascular endothelial cells.Methods Human umbilical vein endothelial cells(HUVEC)were serum-starved and then exposed to HSA from varied sources.Cellular responses were assessed using the CCK-8 assay for proliferation,Annexin V-FITC kit for apoptosis,and flow cytometry for cell cycle analysis.The proliferative,apoptotic and cell cycle effects of HSA from different sources were statistically compared.Additionally,the impact of HSA on HUVEC cell migration and tube formation was assessed via scratch assays and tube formation experiments.Results Yeast-derived rHSA1 did not affect HUVEC proliferation(P=0.49,q=1.601),while other HSA sources significantly promoted it(P＜0.001,F=10.84).All HSA treatment groups exhibited an inhibitory effect on HUVEC apoptosis(P＜0.001),with no statistically significant differences in the proportions of live cells(P=0.07,F=2.415),apoptotic cells(P=0.2,F=1.624),and dead cells(P=0.28,F=1.376)across treatment groups.Compared to the serum-free control group,except for the pHSA2 and pHSA6 treatment groups,which showed a significant decrease in G0/G1 phase cell proportion(P＜0.001)and a significant increase in G2/M phase cell proportion(P＜0.01),the proportions of cells in other cell cycle phases did not show significant changes in the HSA treatment groups.In the scratch assay,in contrast to the serum-free control group,the pHSA(1)and pHSA(2)groups exhibited a higher degree of wound healing at 12 h(P＜0.001),24 h(P＜0.001),and 36 h(P＜0.001,P=0.002);however,no significant differences were observed in the rHSA(1)and rHSA(2)groups compared to the control group.Likewise,in the tube formation assay,the pHSA(1)and pHSA(2)groups significantly enhanced node formation(P=0.001,P=0.005),tubular branching(P＜0.001),and mesh structure development(P＜0.001),whereas the rHSA(1)and rHSA(2)groups showed no such enhancements.Conclusion HSA from different sources significantly inhibits endothelial cell apoptosis under serum-free conditions,but exerts varying effects on proliferation,cell cycle regulation,cell migration,and tube formation of vascular endothelial cells.
Human Serum AlbuminVascular Endothelial CellsProliferationApoptosisMigrationAngiogenesis
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