Analysis and Clinical Significance of Minor Cross-match Incompatibility with Micro-column Gel Test
Objective To analyze the cause and significance of minor cross-match incompatibility by using micro-column gel test(MGT),and to provide reference for clinical transfusion.Methods Samples of transfusion recipients were collected from July 2022 to July 2023 with minor cross-match incompatible while antibody screening test negative.Direct antiglobulin test(DAT)was performed,Positive samples undergoing acid elution test.Antibody screening and identification were conducted on the eluate.DAT-negative individuals underwent donor antibody screening or re-cross-matching.A part of DAT-positive transfused patients were selected as the experimental group,and patients with major and minor cross-match compatibility and negative DAT were selected by propensity score matching as the control group for comparison and evaluation of transfusion efficacy.Results Among 380 patients with major cross-match compatibility and the minor cross-match incompatibility,372 patients were DAT positive(97.89%).Antibody test of the acid elute,364 cases showing negative results(97.85%),6 cases were positive(1.61%)with no pattern.Two cases showed suspected reactive patterns(0.54%),but no specific antibodies were identified.These patients exhibited a significant increase in hemoglobin after receiving red blood cell suspension transfusion(P＜0.05),with no impact on the safety and efficacy of transfusion compared to the control group.Among DAT-negative patients,4 cases were antibody screening positive(1.05%),1 case had gel card abnormalities(0.26%),2 cases were due to human error(0.52%),and 1 case was a false aggregation(0.26%).Conclusion The minor cross-match incompatibility with MGT has almost no impact on the safety and efficacy of transfusion for patients,and the majority of reasons for minor cross-match incompatibility are due to DAT positivity in patients.In addition,a small number of minor cross-match incompatibility were caused by human error,false aggregation and abnormal gel cards.
万方数据
维普
