治疗浓度哇巴因增强大鼠心室肌细胞收缩功能的机制研究
Study on the Mechanism of Enhanced Contractility of Ventricular Myocytes in Rats with Treatment Concentration of Ouabain
周晓辉 1蔡玥 2郭珊珊 1王淑梅 1王娜 1张博 1吴清华 3王玲娇4
作者信息
- 1. 061001 河北 沧州,沧州市人民医院药学部
- 2. 050051 石家庄,河北省人民医院药学部
- 3. 061001 河北 沧州,沧州市中心医院心内六科
- 4. 050031 石家庄,河北医科大学第一医院药学部
- 折叠
摘要
目的 探讨治疗浓度(10-8 mol/L)哇巴因对急性分离大鼠心室肌细胞收缩功能的影响及钠钾泵激活的相关信号转导通路是否参与了治疗浓度哇巴因的强心作用.方法 采用酶解法急性分离大鼠心室肌细胞并复钙,可视化动缘探测系统检测单个大鼠心室肌细胞收缩功能.观察 10-9～10-4 mol/L哇巴因对急性分离大鼠心室肌细胞收缩力的影响.采用10-8 mol/L哇巴因分别与1 μmol/L PP2、100 μmol/L NAC、1 μmol/L U73122、1 μmol/L Xesto-spongin C和50 μmol/L PD98059 共同灌流,观察记录5 种信号转导通路抑制剂对10-8 mol/L哇巴因强心作用的影响.结果 10-9～10-4 mol/L的哇巴因均能使急性分离大鼠心室肌细胞收缩力增强(P＜0.01),且具有浓度依赖性.1 μmol/L PP2、100 μmol/L NAC、1 μmol/L U73122 及 1 μmol/L Xestospongin C均可部分抑制10-8 mol/L哇巴因的强心作用(P＜0.05,P＜0.01);但50 μmol/L PD98059 +10-8 mol/L哇巴因与10-8 mol/L哇巴因使大鼠心室肌细胞收缩幅度比较差异无统计学意义(P＞0.05).结论 10-9～10-4 mol/L的哇巴因均能够增强急性分离大鼠心室肌细胞收缩力.10-8 mol/L哇巴因的强心作用极有可能与钠钾泵激活的Src/EGFR/Ras/ROS信号转导通路和Src/EGFR/PLC/PIP2/IP3 信号转导通路有关.
Abstract
Objective To investigate the effect of treatment concentration(10-8 mol/L)of Ouabain on contractile function of acutely isolated ventricular myocytes of rats and to study whether the signal transduction pathway activated by sodi-um and potassium pump is involved in the cardiac strengthening effect of Ouabain.Methods Ventricular cardiomyocytes of rats were acutely isolated by enzymolysis and supplemented with calcium.The systolic function of single rat cardiomyocytes were detected by visual single-cell moving edge detection system.The effect of 10-9-10-4 mol/L Ouabain on contractility of acutely isolated rat cardiomyocytes was observed.10-8 mol/L Ouabain was co-irrigated with 1 μmol/L PP2,100 μmol/L NAC,1 μmol/L U73122 and1 μmol/L Xestospongin C,and50 μmol/L PD98059,respectively.The effect of 5 kinds of sig-nal transduction pathway inhibitors on cardiotonic effect of 10-8 mol/L Ouabain was observed and recorded.Results The contractility of acutely isolated ventricular myocytes of rats was enhanced by 10-9～10-4 mol/L Ouabain(P＜0.01)in a concentration-dependent manner.1 μmol/L PP2,100 μmol/L NAC,1 μmol/L U73122 and 1 μmol/L Xestospongin C could partly inhibit the cardiotonic effect of 10-8 mol/L Ouabain(P＜0.05,P＜0.01).However,there was no significant differ-ence in the contraction amplitude of ventricular myocytes between 50 μmol/L PD98059 + 10-8 mol/L Ouabain and 10-8 mol/L Ouabain(P＞0.05).Conclusion 10-9 to10-4 mol/L Ouabain can increase the contractility of acutely isolated ventricular myocytes of rats.The cardiotropic effect of 10-8 mol/L Ouabain is most likely related to the Src/EGFR/Ras/ROS signal transduction pathway and the Src/EGFR/PLC/PIP2/IP3 signal transduction pathway activated by the sodium potassium pump.
关键词
哇巴因/强心苷/大鼠/心室肌细胞/收缩功能/钠钾泵/强心/机制Key words
Ouabain/Cardiac glycosides/Rat/Ventricular myocytes/Systolic function/Na+/K+-ATPase/Cardio-tropic effect/Mechanism引用本文复制引用
基金项目
河北省卫健委医学科学研究课题(20200295)
出版年
2023
国家科技期刊平台
NSTL
万方数据