Objective To analyze the effects of high-risk human papillomavirus(HR-HPV)infection on the cervico-vaginal microecology and the expression of miR-21 and cyclin-dependent kinase 5(CDK5)in cervical exfoliated cells.Meth-ods A total of 250 patients with cervicitis treated from January 2020 to December 2022 were selected as research subjects,and were divided into HR-HPV-negative group(n =160)and HR-HPV-positive group(n =90)according to HR-HPV infec-tion.HPV infection subtypes were detected in the HR-HPV-positive group,and cervicovaginal microecology between the two groups was compared.Real-time fluorescence quantitative PCR was used to detect the expression of miR-21 and CDK5 in two groups of cervical exfoliated cells.Spearman method was used to analyze the relationship between HR-HPV infection and the expression levels of miR-21 and CDK5 and cervical microecology,and the relationship between the levels of miR-21 and CDK5 in cervical cells and cervicovaginal microecology.Results In the HR-HPV-positive group,the detection rate of HR-HPV in-fection was the highest with HPV16 subtype,accounting for 53.33％.The rate of vaginal microecological imbalance,positive rate of ureaplasma urealyticum(UU),positive rate of chlamydia,abnormal rate of lactobacillus and incidence of bacterial vaginitis in HR-HPV-positive group were higher than those in HR-HPV-negative group(P<0.01).The relative expression levels of miR-21 and CDK5 in cervical exfoliated cells in HR-HPV-positive group were higher than those in HR-HPV-negative group(P<0.01).The expression levels of miR-21 and CDK5 in cervical exfoliated cells were positively correlated with the incidence of bacterial vaginitis,the positive rate of UU,the abnormal rate of lactobacillus and the positive rate of chlamydia(P<0.01).Conclusion HR-HPV infection can aggravate the disturbance of cervicovaginal microecology,increase the se-verity of cervical lesions,and significantly increase the expression of miR-21 and CDK5 in cervical exfoliated cells.
维普
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