Objective To investigate the correlation between serum miR-182 and miR-21 expression and human pap-illomavirus(HPV)infection in patients with cervical cancer(CC).Methods In total,120 patients who underwent cervical biopsy from April 2020 to April 2023 were selected as the research subjects.Patients with confirmed CC were selected as CC group(n=49),patients with confirmed cervical intraepithelial neoplasia(CIN)as CIN group(n=39),and patients with confirmed cervicitis as cervicitis group(n=32).The serum levels of miR-182 and miR-21 were detected by real-time quanti-tative reverse transcription polymerase chain reaction,and HPV-DNA and HPV E6/E7 mRNA load were detected by polymer-ase chain reaction amplification and second-generation hybridization capture test.Multivariate Logistic regression was used to analyze the factors affecting the occurrence of CC.Spearman method was used to analyze the correlation between serum miR-182 and miR-21 levels and HPV infection in patients with CC.Results The serum miR-182 and miR-21 levels in CC group were higher than those in CIN group and cervicitis group,and the serum miR-182 and miR-21 levels in CIN group were higher than those in cervicitis group(P＜0.05).The positive expression rate of HPV-DNA and the high load rate of HPV E6/E7 mRNA in CC group,CIN group and cervicitis group were decreased successively(P＜0.05).Serum miR-182 and miR-21 levels were positively correlated with HPV-DNA expression and HPV E6/E7 mRNA load in patients with CC(P＜0.01).miR-182,miR-21,HPV-DNA expression,and HPV E6/E7 mRNA load were independent risk factors for CC(P＜0.01).Conclusion The elevated levels of miR-182 and miR-21 in patients with CC are closely related to cervical HPV infection.
维普
万方数据