Objective To explore the diagnostic value and significance of bronchoalveolar lavage fluid(BALF)cul-ture and metagenomic next-generation sequencing(mNGS)detection in community-acquired pneumonia(CAP)/hospital-acquired pneumonia(HAP).Methods Eighty-one patients with CAP/HAP treated from October 2022 to April 2024 were selected.BALF was collected,and microbial culture and mNGS detection were performed.BALF microbial culture and mNGS pathogen detection results were recorded.The detection rates of BALF microbial culture and mNGS detection for different pathogens were compared,and the detection rates of BALF microbial culture and mNGS detection for CAP/HAP type were compared.Results The positive detection rate of mNGS[86.42％(70/81)]was higher than that of BALF microbial cul-ture[22.22％(18/81)],showing significant difference(P<0.05).Of 18 patients with positive BALF microbial culture,26 strains of pathogens were detected,and of 70 patients with positive mNGS detection,148 strains of pathogens were detec-ted.There was no significant difference in the detection rate of different pathogens between BALF microbial culture and mNGS detection(P>0.05).The detection rate of mNGS for both single and mixed CAP/HAP was higher than that of BALF micro-bial culture,with a statistically significant difference(P<0.05).The sequence number of 70 positive samples detected by mNGS was(1 293.51±394.86)bp,with a high abundance of 84.29％(59/70),a medium abundance of 14.29％(10/70),and a low abundance of 1.43％(1/70).The reliability of the results from mNGS in detecting pathogens was high.In 70 patients with positive mNGS detection of CAP/HAP,148 strains of pathogens were detected,mainly bacterial infections(56.76％,84/148),with Gram-negative bacteria being the predominant type(39.86％).Conclusion The value of mNGS detection in diagnosing CAP/HAP is significantly better than that of BALF microbial culture.It can improve the detection rate of different pathogen species and different types of CAP/HAP,thereby guiding targeted antimicrobial treatment in clinical practice.
维普
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