外周血内皮微粒中miR-126水平与MHR在急性心肌梗死患者PCI术后的表达水平及临床意义
Expression levels and clinical significance of miR-126 in circulating endothelia microparticles and the monocyte to high-density lipoprotein ratio in patients with acute myocardial infarction after percutaneous coronary intervention
帕丽达·玉山江 1艾买提江·买买提 2古力斯坦·艾斯卡尔 3尼格热·阿力木 3吐尔孙阿依·依斯提米拉 3穆叶赛·尼加提4
作者信息
- 1. 新疆维吾尔自治区第二人民医院急救中心(乌鲁木齐,830000)
- 2. 喀什地区第二人民医院心内科
- 3. 新疆医科大学
- 4. 新疆维吾尔自治区人民医院急救中心
- 折叠
摘要
目的:分析急性心肌梗死(AMI)患者在接受经皮冠状动脉介入治疗(PCI)后,外周血内皮细胞来源的微粒中的microRNA-126(miR-126)表达水平以及单核细胞与高密度脂蛋白胆固醇比值(MHR)的变化及其临床意义.方法:纳入2019-2022年在新疆维吾尔自治区人民医院心内科进行冠状动脉造影(CAG),随后确诊为AMI并接受PCI治疗的60例患者(AMI组).同时,纳入60例慢性冠状动脉疾病(CCD)患者(CCD组)以及60例健康个体(健康组)作为对照.测定并对比各组患者外周血内皮微粒中的miR-126和MHR表达水平,并计算Gensini评分.相关性分析采用Spearman检验,AMI发生的危险因素采用logistic回归分析,各指标的诊断价值采用ROC曲线分析.结果:AMI组miR-126水平低于CCD组及对照组,MHR高于CCD组及对照组.Spearman相关性分析结果表明,miR-126的表达水平与Gensini评分呈负相关性(r=-0.460,P＜0.001),MHR与 Gensini 评分呈正相关性(r=0.468,P＜0.001).多因素 logistic 回归分析显示,miR-126(OR=6.995,95％CI:2.126～23.018,P=0.001)、MHR(OR=4.527,95％CI:1.145～17.895,P＜0.031)和 Gensini 评分(OR=0.949,95％CI:0.925～0.974,P＜0.001)是AMI发生的独立危险因素.ROC曲线分析表明,miR-126、MHR和Gensini评分联合检测预测AMI的曲线下面积(AUC)为0.894(95％CI:0.951～0.836,P＜0.001),特异度为95％,灵敏度为76.7％,高于各指标单独预测.结论:miR-126水平和MHR与AMI的发生及冠心病严重程度有重要联系,是潜在的预测和诊断AMI的生物标记物.
Abstract
Objective:To analyze the expression levels of microRNA-126(miR-126)in endothelial cell-derived microparticles in peripheral blood and the changes in the ratio of monocyte to high-density lipoprotein cholesterol(MHR)in patients with acute myocardial infarction(AMI)after undergoing percutaneous coronary intervention(PCI),as well as their clinical significance.Methods:Sixty patients who underwent coronary angiography(CAG)and were subsequently diagnosed with AMI,then received PCI treatment at the Department of Cardiology,People's Hospital of Xinjiang Uygur Autonomous Region from 2019 to 2022 were included(AMI group).Addi-tionally,60 patients with chronic coronary disease(CCD)were included in the CCD group,60 healthy individuals were included in the health group.The expression levels of miR-126 in peripheral blood endothelial microparticles,the MHR,and Gensini scores among each group were measured and compared.The correlation analysis was con-ducted using Spearman test,the risk factors for AMI were analyzed using logistic regression,and the diagnostic value of each indicator was analyzed using ROC curve.Results:The miR-126 levels in the AMI group were lower than those in the CCD and control groups,while the MHR was higher than those in the CCD and NC groups.The Spearman correlation analysis indicated that the miR-126 levels was negatively correlated with Gensini scores(r=-0.460,P＜0.001),while MHR was positively correlated with Gensini score(r=0.468,P＜0.001).Multiva-riate logistic regression analysis showed that miR-126(OR=6.995,95％CI:2.126-23.018,P=0.001),MHR(OR=4.527,95％CI:1.145-17.895,P＜0.031),and Gensini score(OR=0.949,95％CI:0.925-0.974,P＜0.001)were independent risk factors for the occurrence of AMI.ROC curve analysis indicated that the area under the curve(AUC)of miR-126,MHR,and Gensini score combined detection for predicting AMI was 0.894(95％CI:0.951-0.836,P＜0.001),with a specificity of 95％and a sensitivity of 76.7％,which was higher than the prediction of each indicator separately.Conclusion:The levels of miR-126 and MHR are significantly as-sociated with the occurrence and severity of coronary heart disease,and they are potential biomarkers for diagno-sing and predicting AMI.
关键词
急性心肌梗死/经皮冠状动脉介入/microRNA-126/单核细胞/高密度脂蛋白胆固醇比值Key words
acute myocardial infarction/percutaneous coronary intervention/microRNA-126/monocyte to high-density lipoprotein cholesterol ratio引用本文复制引用
基金项目
新疆维吾尔自治区自然科学基金计划特配项目(2023D03017)
出版年
2024
NETL
NSTL
万方数据