Cause analysis and countermeasures of 20 cases of inconsistent positive and negative stereotypes by micro-column gel card method
Objective:To investigate the reasons for the inconsistency of ABO positive and negative stereotypes by micro-column gel card method,and explore the corresponding countermeasures.Methods:Serological tests of different blood types were carried out in the cases with inconsistent positive and negative typing by saline medium test tube method:Absorption-elution test,determination of blood group substances in saliva,direct antiglobulin test,screening and identification of irregular antibodies and clinical data were analyzed comprehensively.Results:In 4578 cases of blood type data,20 cases of positive and negative stereotypes were inconsistent.Among them,there were 4 cases of infants and children,4 cases of antigen antibody weakening,3 cases of subtype,2 cases of irregular antibody,2 cases of M protein,2 cases of cold autoantibodies,2 cases of autoimmune hemolytic anemia,and 1 case after ABO incompatible hematopoietic stem cell transplantation.Saline test tube method could correct 7 cases,including 3 cases of infants,2 cases of M protein;and 2 cases of cold autoantibodies.Conclusion:The card method combined with the tube method might be helpful to correct the inconsistency of positive and negative stere-otypes caused by some infants,cold autoantibodies and M protein.For the positive and negative stereotypes that could not be corrected by the test tube method,comprehensive analysis combined with many serological methods of blood group and clinical data of patients,etc.might be conducive to the final determination of blood group.
positive and negative stereotypes inconsistentmicro-column gel card methodsaline test tube methodblood group serology
NETL
NSTL
万方数据
