Anti-D production induced by DEL type blood in Rh(D)negative patients and blood matching incompatibility
To analyze the cause of cross matching incompatibility caused by high titer antibody D after infu-sion of DEL type blood in a Rh(D)negative patient,and provide strategic recommendations for safe blood transfu-sion in Rh(D)negative patients.The irregular antibodies were screened and identified by brine test tube method and microcolumn gel anti human globulin method,and the irregular antibodies were specifically identified and titer determined by microcolumn gel anti human globulin method.The mismatched red blood cells and the patient's ser-um were absorbed at 37℃ and then dissipated at 56℃.The obtained diffusion solution was identified by microcol-umn gel anti human globulin method for antibody specificity.At the same time,the blood donors'red blood cells were detected for DEL type by absorption and diffusion method.The screening result for irregular antibodies in the patient's serum was positive,and the nature of the antibody was IgG type.The specificity of the antibody was anti-D,with a titer of 2 048.The specificity of the red blood cell absorption and release solution antibody was identified as anti-D.After absorption and release testing,the donor was classified as DEL type(a retrospective investigation showed that the red blood cells transfused by this patient 17 days ago were also DEL type).The in-fusion of DEL type blood into Rh(D)negative patients carried a potential risk of inducing the production of anti-D,which could be combined with DEL type red blood cells again to cause incompatibility.For women with a history of blood transfusion,pregnancy or fertility requirements and patients with blood transfusion dependence,transfu-sion should be cautious.As for those who have already developed anti-D,transfusion should only be performed in emergency situations to avoid the occurrence of hemolytic transfusion adverse reactions,and improve the safety and effectiveness of blood transfusion.
DEL typeanti-Dabsorption and dissipation testRh blood typecross matching
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