Expression and clinical significance of TIGIT,CD226,CD96,and CD155 in platelet glycoprotein autoantibody-positive primary immune thrombocytopenia
Objective:To investigate the expression of TIGIT,CD226,CD96 and CD155 on CD8+T cells and dendritic cells(DC)in the peripheral blood of patients with platelet glycoprotein autoantibody(GPs)-positive pri-mary immune thrombocytopenia(ITP)and to analyze their clinical significance.Methods:Forty-one GP-positive ITP patients and 20 healthy individuals were selected.The flow cytometry was used to detect the percentage of TIGIT,CD226,and CD96 expression on CD8+T cells and the changes in DC cell subsets and the percentage of CD155 expression on plasma DC(pDC)were analyzed.Results:The percentage of pDC and CD155+pDC in the GPs-positive ITP group were significantly higher than those in the healthy control group,and they were signifi-cantly higher in the GP I b/Ⅸ+/Ⅱ b/Ⅲ a+/-group than those in the GP Ⅰ b/Ⅸ-/11 b/Ⅲ a+group(P＜0.05,F=40.83;P＜0.01,F=59.50).On the other hand,the percentage of TIGIT+CD8+T cells in the GPs-positive ITP group was significantly lower than that in the healthy control group,and it was significantly lower in the GP Ⅰ b/Ⅸ+/Ⅱ b/Ⅲ a+/-group than that in the GP Ⅰ b/Ⅸ-/Ⅱ b/Ⅲ a+group(P＜0.05,F=36.41).However,the per-centages of CD226+CD8+T cells and CD96+CD8+T cells were significantly higher than those in the control group,and they were significantly higher in the GP Ⅰ b/Ⅸ+/Ⅱ b/Ⅲ a+/-group than those in the GP Ⅰ b/Ⅸ-/Ⅱ b/Ⅲa+group(P＜0.05,F=45.01;P＜0.01,F=41.66).The percentages of CD155+pDC in the GP Ⅰ b/Ⅸ-/Ⅱ b/Ⅲa+group and GP Ⅰ b/Ⅸ+/Ⅱ b/Ⅲ a+/-group were negatively correlated with TIGIT+CD8+T cells(P＜0.01,r=-0.54;P＜0.01,r=-0.76),while it positively correlated with the percentages of CD226+CD8+T cells and CD96+CD8+T cells(P＜0.05,r=0.61;P＜0.05,r=0.51;P＜0.01,r=0.79;P＜0.01,r=0.73).Conclusion:The abnormal expression of TIGIT,CD226,CD96,and CD155 contributes to immune in-tolerance in GPs-positive ITP,which may be involved in the pathogenesis of the disease.
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