首页|Tfh细胞上TIGIT和PD-1共表达在慢性淋巴细胞白血病患者外周血中的变化特点及意义

Tfh细胞上TIGIT和PD-1共表达在慢性淋巴细胞白血病患者外周血中的变化特点及意义

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目的:初步探讨慢性淋巴细胞白血病(chronic lymphocytic leukemia,CLL)患者外周血共抑制分子T 细胞免疫球蛋白和 ITIM 结构域(T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain,TIGIT)和程序性细胞死亡受体l(programmed cell death protein 1,PD-1)在Tfh细胞上的表达变化特点及临床意义.方法:采用流式细胞术检测50例CLL患者、36例非CLL的慢性B淋巴细胞增殖疾病患者和30例健康者外周血Tfh细胞百分比和Tfh细胞上PD-1和TIGIT的水平,比较3组受试者的差异.分析CLL患者TIGIT+PD-1+Tfh细胞百分比与IPI评分分层的关系,以及与免疫球蛋白的相关性.结果:①与对照组比较,初诊 CLL 患者 Tfh、PD-1+Tfh、TIGIT+Tfh、TIGIT+PD-1+Tfh、TIGIT+PD-1-Tfh 和 TIGIT-PD-1+Tfh 细胞百分比明显增高,差异有统计学意义(P＜0.05).②TIGIT+PD-1+Tfh细胞在CLL-IPI预后分层低危组、中危组、高危组、极高危组比较分析结果,组间和组内两两比较差异均有统计学意义(P＜0.05),随着预后分层增加TIGIT+PD-1+Tfh细胞水平逐渐增加.③受试者工作特征曲线结果显示,TIGIT+PD-1+Tfh细胞在CLL患者和非CLL患者的鉴别诊断中具有一定的诊断价值(P＜0.05).④Logistic回归分析显示,TIGIT+PD-1+Tfh细胞百分比是CLL发病的独立危险因素(P＜0.05).⑤CLL患者外周血中TIGIT+PD-1+Tfh与IgA、IgM、IgG均呈负相关(P＜0.05).结论:初诊CLL患者外周血TIGIT+PD-1+Tfh细胞百分比明显增加,Tfh细胞高表达共抑制分子TIGIT和PD-1参与CLL疾病的发生,并且与疾病的免疫球蛋白水平降低有关.
The changes and significance of TIGIT and PD-1 co-expression on Tfh cells in peripheral blood of patients with chronic lymphocytic leukemia
Objective:To investigate the expression and clinical significance of T-cell immunoglobulin and immunore-ceptor tyrosine-based inhibitory motif domain(TIGIT)and programmed cell death protein 1(PD-1)on T follicular helper cells(Tfh)in peripheral blood of patients with chronic lymphocytic leukemia(CLL).Methods:The percentage of periph-eral blood Tfh cells and the levels of PD-1 and TIGIT were measured by flow cytometry in 50 CLL patients,36 chronic B lymphocyte proliferation disease patients without CLL,and 30 healthy individuals.The differences among the three groups of subjects were compared.The percentage of TIGIT+PD-1+Tfh cells in CLL patients was analyzed in relation to IPI score stratification,as well as correlation with immunoglobulins.Results:①Compared with healthy controls,the percentages of Tfh,PD-1+Tfh,TIGIT+Tfh,TIGIT+PD-1+Tfh,TIGIT+PD-1-Tfh and TIGIT PD-1+Tfh cells were significantly higher in newly diagnosed CLL patients(P＜0.05).②The results of comparative analysis of TIGIT+PD-1+Tfh cells in CLL-1P1 prognostic stratification low-risk,intermediate-risk,high-risk,and very high-risk groups showed that there were significant differences between and within groups(P＜0.05),and TIGIT+PD-1+Tfh cell levels gradually increased with increasing prognostic stratification.③ The results of receiver operating characteristic curve showed that TIGIT+PD-1+Tfh cells had certain diagnostic value in the differential diagnosis of CLL patients and non-CLL patients(P＜0.05).④Logistic regression analysis showed that the percentage of TIGIT+PD-1+Tfh cells was an independent risk factor for CLL(P＜0.05).⑤ TIGIT+PD-1+Tfh was negatively correlated with IgA,IgM,and IgG levels in the peripheral blood of CLL patients(P＜0.05).Conclusion:The percentage of TIGIT+PD-1+Tfh cells in pe-ripheral blood of newly diagnosed CLL patients is significantly increased.TIGIT and PD-1,which are highly expressed in Tfh cells,are involved in the occurrence of CLL diseases and are associated with decreased immunoglobulin levels in the diseases.

chronic lymphocytic leukemiaT follicular helper cellprogrammed cell death protein 1T-cell immu-noglobulin and immunoreceptor tyrosine-based inhibitory motif domain

张瑞、骆婷婷、陈双、曲建华

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新疆医科大学第一附属医院血液病中心实验室(乌鲁木齐,830054)

新疆维吾尔自治区血液病研究所

慢性淋巴细胞白血病 滤泡辅助性T细胞 程序性细胞死亡受体1 T细胞免疫球蛋白和ITIM结构域

新疆维吾尔自治区天山创新团队项目

2022D14008

2024

10.13201/j.issn.1004-2806.2024.05.012

临床血液学杂志
华中科技大学同济医学院血液病研究所 北京医科大学血液病研究所

临床血液学杂志

CSTPCD
影响因子：1.063
ISSN：1004-2806
年,卷(期)：2024.37(5)
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