Identification and analysis of mimicking anti-E autoantibodies and exploration of blood transfusion strategy
Objective:To identify and analyze irregular antibodies in autoantibody patients,explore the detec-tion methods of antibody-like antibodies,and provide reference for formulating transfusion strategies and guiding clinical rational use of blood.Methods:A patient with difficult cross-matching blood was admitted in our laborato-ry.The ABO and Rh blood type,antibody screening,antibody specificity identification,direct anti-human globu-lin test,absorption and diffusion test,antibody titer and cross-matching blood test were performed by test tube method.The new and old red blood cells were separated by capillary ultra-centrifugal method,and Rh blood type was identified.Results:The patient's blood type was B and Rh blood type was CcDEe.Both direct antiglobulin test and self-controlled test were positive.Serum antibody screening by saline test tube was negative,but Liss-IAT test was positive,after absorption of red blood cell(ccee),the supernatant of red blood cell was negative with red blood cell(ccee,CCEe).The reaction between the diffusion solution and erythrocyte(ccee)was negative,the reac-tion with erythrocyte(CCEe)was weakly positive,after absorption of autoerythrocyte,the serum antibody screen-ing test was negative.Cross matching test with Erythrocytes of ee blood type was negtive.Conclusion:There were mimicking anti-E autoantibodies in the plasma of the patients.Infusion of red blood cell(ee)could improve the transfusions effect.
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