患者首次输血后短期内产生不规则抗体致血型正反定型不一致分析
Analysis on the forward and reverse blood typing inconsistency caused by irregular antibodies after patients'first blood transfusion in the short term
贲燕华 1王峻霞 1夏和凤 1韩霜1
作者信息
- 1. 东台市人民医院输血科(江苏东台,224200)
- 折叠
摘要
目的:分析患者首次输血后短期内产生不规则抗体对血型正反定型的影响,正确鉴定患者血型和不规则抗体特异性,保证临床输血安全.方法:采用试管法、凝聚胺法、微柱凝胶卡式法进行不规则抗体筛选、抗体鉴定试验、Rh分型及直接抗人球蛋白试验分析不规则抗体特异性;2-巯基乙醇(2-ME)处理标本后鉴定血型.结果:患者血型为B型Rh阳性,Rh分型为ccDEE,血清中检出IgM、IgG抗-C.结论:患者首次输血后即可产生具有临床意义的特异性不规则抗体,建议对献血者和患者进行Rh血型系统抗原(CcEe)分型,可以快速匹配抗原相合血液,避免因抗体筛查阳性引起交叉配血困难而延误患者的输血治疗,减少不规则抗体引起的溶血性输血反应的发生.
Abstract
Objective:To analyze the impact of irregular antibodies produced by patients on forward and re-verse typing among short term after their first blood transfusion,and accurately identify the patient's blood type and the specificity of irregular antibodies,as well as ensure clinical blood transfusion safety.Methods:Test-tube,polyamine and microcolumn-gel-card methods were applied for irregular antibody screening,antibody identifica-tion,Rh typing and direct-anti-human-globulin test,to analyze the specificity of irregular antibodies;blood type was identified after 2-ME treatment.Results:The patient's blood type was B type Rh positive,Rh subtype was ccDEE,and IgM and IgG anti C were detected in the serum.Conclusion:After the first blood transfusion,specific irregular antibodies with clinically significance were produced by patients.It was recommended to perform group system antigen(CcEe)typing on Rh blood for blood-donors and patients,that could make quick blood matching with common antigen,avoid transfusion treatment delay due to difficulty from cross matching caused by positive antibody screening,and reduce the occurrence of hemolytic transfusion reactions caused by irregular antibodies.
关键词
不规则抗体/抗-C/IgG/IgMKey words
irregular antibody/anti-C/IgG/IgM引用本文复制引用
出版年
2024
NETL
NSTL
万方数据