Serological characteristics and gene sequence analysis of ABO variant B305 subtype
Objective:To explore and analyze the serological characteristics and gene sequence of the ABO va-riant B305 subtype and propose strategies for subsequent blood transfusions.Methods:ABO blood group typing was performed using the saline test tube agglutination method.B antigen genotyping was conducted using the PCR-SBT method,and B antigen gene sequencing was carried out using the Sanger sequencing method.Cross-matching was performed using the polybrene method.Results:Three probands exhibited weak B antigens on their red blood cells and had anti-A antibodies in their serum.The allele result was identified as ABO*B3.05,and a c.425 T＞C mutation was detected compared to the standard sequence.This mutation resulted in the substitution of amino acid P.Met142Thr in the polypeptide chain.Cross-matching experiments with type A red blood cells dis-played agglutination reactions,while no agglutination or hemolysis reactions were observed with type B and type O red blood cells.Conclusion:The weak expression of the B305 subtype antigen may be attributed to the ABO blood group exon 7 c.425 T＞C mutation.These findings hold significant implications for the study of subsequent blood transfusion strategies.
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