Analysis of sequence of FUT1 and FUT2 and transfusion scheme of rare para-bombay blood type individuals
Objective:To analyze the serological characteristics and FUT1 and FUT2 sequences of Para-Bom-bay Blood Type individuals,and explore its molecular mechanism and transfusion scheme.Methods:Six suspected para-Bombay phenotype individuals found from January 2019 to December 2023 were collected.ABO positive and negative typing and H antigen were detected by conventional saline tube method,weak A and weak B antigens were detected by absorption and emission test,genotyping was performed by PCR-SBT method,and gene sequen-cing was performed by Sanger detection technology.Results:Six individuals were all para-Bombay blood group,including 2 cases of Amh and 4 cases of Bmh.FUT1 genotype:3 cases of c.551-552delAG,1 case of c.551-552delAG/c.881-882delTT,1 case of c.551-552delAG/c.755 G＞C,and 1 case of c.551-552delAG/c.658 C＞T;FUT2 genotype:5 cases of c.357 C＞T,and 1 case of c.716 G＞A.Conclusion:Molecular diagnosis can accu-rately identify para-Bombay blood group,and special transfusion scheme can ensure safe blood transfusion.
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