Study on the mechanism of Sinomenine inhibiting the growth of Retinoblastoma cells
Objective To explore the mechanism of Sinomenine inhibiting the growth of Retinoblastoma line WERI-RB-1.Methods Immunofluorescence,CCK-8 and flow cytometry were used to analyze the effect of Sinomenine on the viability and apoptosis of WERI-RB-1 cells,and Western blot was used to detect the expression of related proteins.Re-sults The expression of autophagy related proteins Beclin1 and LC3B was up-regulated and p62 was down regulated in WERI-RB-1 cells treated with different concentrations of Sinomenine.After 24 h stimulation with 50 μmol/L Sinomenine and autophagy inhibitor CQ,the increased Beclin1 and LC3B stimulated by Sinomenine recovered,and the decreased p62 protein also recovered;The autophagy stimulated by Sinomenine was reduced(LC3B labeled positive cells were detected by immunofluorescence);It partially alleviated the cell viability reduction induced by Sinomenine(CCK-8 method);The increased apoptosis protein Bax and Bcl-2 stimulated by Sinomenine recovered.Sinomenine inhibits the PI3K/Akt/mTOR pathway,and after activating the PI3K/Akt/mTOR pathway,the effect of Sinomenine on cell growth and autophagy is offset.Conclusions Sinomenine can inhibits retinoblastoma cell growth by promoting autophagy via inactivation of PI3K/Akt/mTOR axis in vitro.
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