Significance of screening differentially expressed miRNA in urine exosomes of children with Kawasaki disease
Objective To study the differential expression of microRNA(miRNA)in urine exosomes of children with Kawasaki disease(KD),and to explore its possibility as a new molecular marker for KD diagnosis.Methods A total of 15 children with KD who were hospitalized in the pediatrics department of the Xi'an No.3 Hospital from January to December 2022 were selected as experimental group,and 15 normal children who underwent physical examination in the child health care department during the same period were selected as control group.Exosomes were extracted from 3 mid-morning urine samples of the two groups by reagent method;high-throughput sequencing analysis was used to detect differentially expressed miRNA in urine exosomes of the two groups,and GO and KEGG pathway analysis were performed.The differential expression of miRNA between the two groups was compared.Results A total of 29 up-regulated differentially expressed miRNA were screened.GO enrichment analysis showed that the biological process(BP)involved in differential miRNA included signal transduction,DNA transcription regulation,RNA polymerase Ⅱ transcription regulation,cell differentiation and phosphorylation process,e tc.;cellular component(CC)included cell membrane,cytoplasm and nucleus,etc.;molecular function(MF)included protein binding,metal ion binding and DNA binding,etc.KEGG pathway analysis showed that the signaling pathways significantly enriched by differential miRNA were cytokine-cytokine receptor interaction,ligand-receptor interaction,material transport and catabolism,intracellular signal transduction,tumor and virus infection,etc.Conclusion The differentially expressed miRNA in urine exosomes can be used as a new molecular marker for the diagnosis of KD.
NETL
NSTL
万方数据
维普
