摘要
目的 通过网络药理学浅究湿润烧伤膏(MEBO)治疗糖尿病足(DF)的作用机制.方法 通过中药系统药理学分析平台(TCMSP)数据库获取MEBO的活性化学成分及有效成分靶点信息,将获得靶点通过UniProt数据库统一基因名称;通过人类基因综合数据库(GeneCards)查询DF的相关靶标基因,利用Venny2.1 将MEBO药物活性成分靶点与DF靶点相映射,获得潜在作用靶点.将交集靶点导入STRING网站进行分析,并使用Cytoscape3.10.0 构建蛋白质-蛋白质相互作用(PPI)网络图,选出关键靶点基因.将交集靶点导入Metascape进行GO和KEGG通路富集分析,并通过Cytoscape软件构建"药物-成分-靶点"网络图.结果 MEBO治疗DF的主要化学成分是小檗碱、汉黄芩素、黄麻苷A、黄连碱、黄柏酮等;关键作用靶点为表皮生长因子受体(EGFR)、一氧化氮合酶3(NOS3)、蛋白激酶B1(AKT1)、白细胞介素-1B(IL-1B)、白细胞介素-6(IL-6)、肿瘤坏死因子(TNF)等;关键作用通路为AGE-RAGE、HIF-1、VEGF、NF-κB等信号通路.结论 本研究初步揭示了MEBO的有效成分可以从多靶点、多通路治疗DF,发挥着改善微环境、抑制创面感染及促进组织修复再生等作用.
Abstract
Objective To explore the mechanism of moist exposed burn ointment(MEBO)in the treatment of diabetic foot(DF)through network pharmacology.Methods The active chemical constituents and active ingredient target information of MEBO were obtained through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the target gene name was obtained through UniProt database;the target genes related to DF were queried through Human Gene Comprehensive Database(GeneCards),and the target of MEBO active ingredient was mapped with the target of DF by Venny2.1 to obtain the potential target of action.The intersection targets were imported into the STRING website for analysis,and the protein-protein interaction(PPI)network map was constructed using Cytoscape3.10.0 to select key target genes.The intersection targets were imported into the GO and KEGG pathway enrichment analysis.The"drug-ingredient-target"network mdp was constructed using Cytoscape software.Results The main chemical components of MEBO in the treatment of DF were berberine,wogonin,juteoside A,coptisine,obacunone,etc.The key role targets were epidermal growth factor receptor(EGFR),nitric oxide synthase 3(NOS3),protein kinase B1(AKT1),interleukin-1B(IL-1B),interleukin-6(IL-6),tumor necrosis factor(TNF),etc.The key role pathways were AGE-RAGE,HIF-1,VEGF,NF-κB and other signaling pathways.Conclusion This study initially revealed that the active ingredients of MEBO can treat DF from multiple targets and multiple pathways,play a role in improving the microenvironment,inhibiting wound infection and promoting tissue repair and regeneration.
维普
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