Network pharmacological study on molecular mechanism of action of Zhitong Xiaoyan ointment in the treatment of chronic eczema
Objective To investigate the molecular mechanism of action of Zhitong Xiaoyan ointment in the treatment of chronic eczema based on network pharmacology.Methods The potential active ingredients of Zhitong Xiaoyan ointment were screened through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrated Database(TCMID)and PubChem database[oral bioavailability(OB)≥30%,drug-likeness(DL)≥0.18].The SwissTargetPrediction database was adopted to predict its action target,and it was mapped with the chronic eczema disease targets obtained from the Disgenet database,GeneCards database,Therapeutic Target Database(TTD),so as to obtain the potential targets of Zhitong Xiaoyan ointment in the treatment of chronic eczema.Cytoscape3.7.2 software was used to construct the Zhitong Xiaoyan ointment-active ingredients-target point network diagram,protein-protein interaction(PPI)network diagram was constructed,and GO functional analysis and KEGG pathway enrichment analysis were performed on the targets of Zhitong Xiaoyan ointment by Bioconductor software package in R software.Results There were 186 potential active ingredients in Zhitong Xiaoyan ointment,and 115 common drug-disease targets were obtained after intersection of Zhitong Xiaoyan ointment and eczema action targets.Sennoside,angelica ether,byakangelicol,rhein,rheinoside,rosmarinic acid,etc.,might be the main active components of Zhitong Xiaoyan ointment in the treatment of chronic eczema,which acted on targets such as TNF,IL-6,AKT1,CXCL8,IL-2,STAT3,and participated in transcriptional regulation,drug reaction and other biological processes and could be used to treat chronic eczema through Kaposi sarcoma-associated herpesvirus infection,TNF signaling pathway,Toll-like receptor signaling pathway and C-type lectin receptor signaling pathway.Conclusion Sennoside,angelica ether,byakangelicol,rhein,rheinoside,rosmarinic acid,etc.are of great significance for Zhitong Xiaoyan ointment in the treatment of chronic eczema;regulating the inflammatory pathway and intervening in the occurrence and development of inflammation may be the mechanism of action of Zhitong Xiaoyan ointment in the treatment of chronic eczema.
万方数据
维普
