摘要
目的 探究类风湿性关节炎(RA)滑膜组织中GATA1表达水平及其与基质金属蛋白酶(MMPs)的相关性.方法 将97例接受关节置换术治疗的RA患者作为观察组,35例因外伤置换关节或截肢的患者作为对照组.比较两组滑膜组织中GATA1、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶-13(MMP-13)表达水平;分析RA滑膜组织炎性浸润情况与GATA1、MMP-1、MMP-3、MMP-9、MMP-13水平的相关性;分析RA患者滑膜组织中GATA1与MMP-1、MMP-3、MMP-9、MMP-13水平的相关性.结果 观察组滑膜组织的GATA1、MMP-1、MMP-3、MMP-9、MMP-13水平高于对照组(P＜0.05).低度滑膜炎患者41例,高度滑膜炎患者56例;高度滑膜炎患者滑膜组织的GATA1、MMP-1、MMP-3、MMP-9、MMP-13水平高于低度滑膜炎患者(P＜0.05).RA患者滑膜组织中GATA1水平与MMP-1、MMP-3、MMP-9、MMP-13水平呈显著正相关(P＜0.05).结论 RA滑膜组织中GATA1呈高表达状态,与组织炎性浸润程度密切相关,其可能通过上调MMP-1、MMP-3、MMP-9、MMP-13表达,参与RA病理发生、发展过程.
Abstract
Objective To investigate the expression level of GATA1 in synovial tissue of rheumatoid arthritis(RA)and its correlation with matrix metalloproteinase(MMPs).Methods A total of 97 patients with RA who underwent joint replacement were included in observation group,and 35 patients who underwent joint replacement or amputation due to trauma were included in control group.The expression levels of GATA1,matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-3(MMP-3),matrix metalloproteinase-9(MMP-9)and matrix metalloproteinase-13(MMP-13)in synovial tissue were compared between the two groups;the correlation between the inflammatory infiltration of RA synovial tissue and the levels of GATA1,MMP-1,MMP-3,MMP-9 and MMP-13 was analyzed;the correlation between GATA1 and MMP-1,MMP-3,MMP-9,MMP-13 levels in synovial tissue of RA patients was analyzed.Results The levels of GATA1,MMP-1,MMP-3,MMP-9 and MMP-13 in synovial tissue of the observation group were higher than those of the control group(P＜0.05).There were 41 patients with low-grade synovitis and 56 patients with high-grade synovitis;the levels of GATA1,MMP-1,MMP-3,MMP-9 and MMP-13 in synovial tissue of the patients with high-grade synovitis were higher than those of the patients with low-grade synovitis(P＜0.05).The level of GATA1 in synovial tissue of RA patients was positively correlated with the levels of MMP-1,MMP-3,MMP-9 and MMP-13(P＜0.05).Conclusion GATA1 is highly expressed in RA synovial tissue,which is closely related to the degree of tissue inflammatory infiltration.It may be involved in the pathogenesis and development of RA by upregulating the expression of MMP-1,MMP-3,MMP-9 and MMP-13.
基金项目
西安市卫生和计划生育委员会2019年卫生科研人才项目(J201901011)
维普
万方数据