首页|饮食干预对妊娠期糖尿病小鼠子代糖尿病易感性的影响

饮食干预对妊娠期糖尿病小鼠子代糖尿病易感性的影响

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目的 探究饮食干预对妊娠期糖尿病(GDM)小鼠子代糖尿病易感性的影响。方法 选取72 只3周龄健康雌性小鼠，并将其随机均分成GDM组和低脂(LF)组。GDM组以饲喂高脂(HF)饲料建立GDM小鼠模型，LF组则饲喂LF饲料。两组小鼠的子代在3周龄断奶后进行饮食干预，分别饲喂HF饲料和LF饲料，并分为GDM HF(G-HF)组、GDM LF(G-LF)组、LF HF(L-HF)组、LF LF(L-LF)组。干预后测其体重、空腹血糖(FBG)，并进行胰岛素耐受性试验(ITT)。结果 HF饮食诱导建立的GDM小鼠模型成模率为 33。33%，此模型发病过程与人类患GDM的病理生理发展过程较为相似。饮食干预到 6 周时，G-HF组的体重高于其他三组，且L-HF组体重高于L-LF组(P<0。05);饮食干预到 12 周时，G-HF组体重高于其他三组(P<0。05)。饮食干预到 6 周时，G-HF组的FBG水平高于G-LF组、L-LF组，L-HF组的FBG水平高于L-LF组(P<0。05);饮食干预到12周时，G-HF组的FBG水平高于其他三组，L-HF组的FBG水平高于L-LF组(P<0。05)。7周龄时，L-LF组对胰岛素的敏感性要高于G-HF组。结论 GDM子代有明显的糖代谢紊乱及体重增加，且LF饮食可降低GDM子代患糖尿病的风险。
Effect of dietary intervention on the susceptibility of offspring diabetes in gestational diabetes mellitus mice
Objective To explore the effect of dietary intervention on the susceptibility of offspring diabetes in gestational diabetes mellitus(GDM)mice.Methods Seventy-two 3-week-old healthy female mice were selected and randomly divided into GDM group and low-fat(LF)group.The GDM group was fed with high-fat(HF)diet to establish a GDM mouse model,and the LF group was fed with LF diet.The offspring of the two groups of mice were fed with HF diet and LF diet after weaning at 3 weeks of age,and were divided into GDM HF(G-HF)group,GDM LF(G-LF)group,LF HF(L-HF)group and LF LF(L-LF)group.After intervention,the body weight and fasting blood glucose(FBG)were measured,and insulin tolerance test(ITT)was conducted.Results The success rate of GDM mouse model induced by HF diet was 33.33%,the pathogenesis of this model was similar to the pathophysiological development of human GDM.At 6 weeks of dietary intervention,the body weight of the G-HF group was higher than that of the other three groups,and the body weight of the L-HF group was higher than that of the L-LF group(P<0.05);after 12 weeks of diet intervention,the body weight of the G-HF group was higher than that of the other three groups(P<0.05).After 6 weeks of diet intervention,the FBG level of the G-HF group was higher than that of the G-LF group and the L-LF group,and the FBG level of the L-HF group was higher than that of the L-LF group(P<0.05);at 12 weeks of diet intervention,the FBG level of the G-HF group was higher than that of the other three groups,and the FBG level of the L-HF group was higher than that of the L-LF group(P<0.05).At 7 weeks of age,the sensitivity of the L-LF group to insulin was higher than that of the G-HF group.Conclusion GDM offspring have obvious glucose metabolism disorder and weight gain,and LF diet can reduce the risk of diabetes in GDM offspring.

dietary interventiongestational diabetes mellitusbody weightfasting blood glucoseinsulin tolerance test

林静、毛榕榕、闵颖俊、李通芬、金会艳

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昆明医科大学第二临床医学院,云南 昆明,650106

昆明医科大学病理学与病理生理学系,云南 昆明, 650500

昆明医科大学第六附属医院儿科,云南 玉溪,653100

昆明医科大学基础医学院实验教学中心机能学实验室,云南 昆明,650500

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饮食干预 妊娠期糖尿病 体重 空腹血糖 胰岛素耐受性试验

2020年度云南省科技厅-昆明医科大学应用基础研究联合专项资金项目昆明医科大学2020年省级大学生创新训练计划项目昆明医科大学大学生创新性实验项目

202001AY070001-0162020106780492020JXD022

2024

10.19347/j.cnki.2096-1413.202407008

临床医学研究与实践

临床医学研究与实践

ISSN：
年,卷(期)：2024.9(7)
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