Objective To explore the effect of dietary intervention on the susceptibility of offspring diabetes in gestational diabetes mellitus(GDM)mice.Methods Seventy-two 3-week-old healthy female mice were selected and randomly divided into GDM group and low-fat(LF)group.The GDM group was fed with high-fat(HF)diet to establish a GDM mouse model,and the LF group was fed with LF diet.The offspring of the two groups of mice were fed with HF diet and LF diet after weaning at 3 weeks of age,and were divided into GDM HF(G-HF)group,GDM LF(G-LF)group,LF HF(L-HF)group and LF LF(L-LF)group.After intervention,the body weight and fasting blood glucose(FBG)were measured,and insulin tolerance test(ITT)was conducted.Results The success rate of GDM mouse model induced by HF diet was 33.33%,the pathogenesis of this model was similar to the pathophysiological development of human GDM.At 6 weeks of dietary intervention,the body weight of the G-HF group was higher than that of the other three groups,and the body weight of the L-HF group was higher than that of the L-LF group(P<0.05);after 12 weeks of diet intervention,the body weight of the G-HF group was higher than that of the other three groups(P<0.05).After 6 weeks of diet intervention,the FBG level of the G-HF group was higher than that of the G-LF group and the L-LF group,and the FBG level of the L-HF group was higher than that of the L-LF group(P<0.05);at 12 weeks of diet intervention,the FBG level of the G-HF group was higher than that of the other three groups,and the FBG level of the L-HF group was higher than that of the L-LF group(P<0.05).At 7 weeks of age,the sensitivity of the L-LF group to insulin was higher than that of the G-HF group.Conclusion GDM offspring have obvious glucose metabolism disorder and weight gain,and LF diet can reduce the risk of diabetes in GDM offspring.
关键词
饮食干预/妊娠期糖尿病/体重/空腹血糖/胰岛素耐受性试验
Key words
dietary intervention/gestational diabetes mellitus/body weight/fasting blood glucose/insulin tolerance test
维普
万方数据