Application progress of metagenomic next-generation sequencing in smear negative pulmonary tuberculosis
Tuberculosis is a public health problem of global concern.The number of global tuberculosis,drug-resistant tuberculosis and tuberculosis deaths is gradually increasing,and the situation of tuberculosis in China is not optimistic.Early and accurate etiological diagnosis is the key to effective treatment,but most of the tuberculosis patients in China are negative or lack of etiological test results.For pathogenic negative pulmonary tuberculosis,the current commonly used pathogenic detection methods can not meet the clinical diagnostic needs.The emergence of metagenomic next-generation sequencing(mNGS)has subverted the traditional tuberculosis detection technology and etiological diagnosis method,and provided a reliable reference for the early diagnosis and early treatment of smear negative pulmonary tuberculosis.This article reviews the existing detection methods of Mycobacterium tuberculosis(MTB)and the application of mNGS technology at home and abroad,and focuses on the advantages and limitations of mNGS in the diagnosis of smearnegative pulmonary tuberculosis,so as to provide reference for clinical effective diagnosis of smear negative pulmonary tuberculosis and rational application of mNGS.
万方数据
维普
