Study on oral flora in patients with early esophageal cancer
Objective To analyze the composition and difference of oral salivary flora between patients with early esophageal cancer and healthy people,and to explore the relationship between the occurrence of early esophageal cancer and oral salivary flora,so as to provide potential markers for the screening of early esophageal cancer.Methods The saliva samples of 39 patients with early esophageal cancer(early esophageal cancer group)and 51 healthy people(healthy control group)were collected from January 2018 to January 2019.The total DNA was extracted and the Illumina sequencing experiment process was adopted.The sequencing data were subjected to operational taxonomic units(OTU)clustering,species composition analysis,diversity analysis and species difference analysis.Results A total of 3 012 385 sequences were obtained from the two groups of saliva samples,and the average effective sequence number was 33 471.The cluster OTU of the early esophageal cancer group was(378±48),and the cluster OTU of the healthy control group was(345±53).Species analysis showed that,the colony composition of the two groups of saliva flora was basically the same,and the diversity difference was small,but the relative abundance was different.Compared with the healthy control group,the abundance ratios of Firmicutes,Bacteroidetes and Fusobacteria at the phylum level in the early esophageal cancer group increased,while the proportion of Proteobacteria decreased significantly.The abundance ratios of Pre votella,Streptococcus and V eillonella at the genus level increased,while the proportion of Neisseria decreased significantly.There was no significant difference in Shannon and Simpson index between the early esophageal cancer group and the healthy control group(P>0.05).Conclusion Compared with the healthy control group,the abundance of Firmicutes,Bacteroidetes and Fusobacteria in the saliva of the early esophageal cancer group was relatively increased,while the abundance of Proteobacteria and Neisseria was relatively reduced.It is speculated that there may be a certain relationship between the imbalance of oral saliva flora and the incidence of early esophageal cancer.
万方数据
维普
