Anti-inflammatory and anti-adhesion effects of the No.2 Gao's Penyan prescription in rats with chronic pelvic inflammatory disease and its influences on the expression of ICAM-1 and caspase-8
Objective To investigate the anti-inflammatory and anti-adhesion effects of the No.2 Gao's Penyan prescription in rats with chronic pelvic inflammatory disease(CPID)and its influences on the expression of intercellular cell adhesion molecule-1(ICAM-1)and caspase-8.Methods Firstly,the minimum inhibitory concentration(MIC)of the No.2 Gao's Penyan prescription was observed by in vitro antibacterial test.Seventy-five female SD rats were randomly divided into blank group(n=10)and modeling group(n=65).After 10 d of modeling,5 rats were randomly selected from the modeling group and sacrificed,after HE staining of uterine tissue,chronic inflammatory changes were observed under the microscope,suggesting that the modeling was successful.Subsequently,60 rats were randomly divided into model group,Chinese medicine group,Chinese patent medicine group and human placental tissue fluid group,the rats of the Chinese medicine group were divided into low,medium and high dose groups,with 10 rats in each group.The model group was given 2 mL of normal saline by gavage,the low,medium and high dose groups of Chinese medicine were given 7,14 and 28 g/kg of the No.2 Gao's Penyan prescription,the Chinese patent medicine group was given 3 g/kg of Puling Penyankang granules by gavage,the human placenta tissue fluid group was given 1 mL/kg of human placenta tissue fluid by intraperitoneal injection.Each of the above administration groups was administered once a day for 20 consecutive days.The rats were sacrificed 24 h after the last administration,and the uterine tissue was weighed,the uterine swelling degree and uterine coefficient were calculated;the pathological changes of rat uterus were observed under light microscope;the expression levels of ICAM-1 and caspase-8 protein in rat uterine tissue were detected by immunohistochemistry and Western-blot.Results The No.2 Gao's Penyan prescription had obvious antibacterial effect on Staphylococcus aureus,Escherichia coli and beta hemolytic Streptococcus.Compared with the blank group,the uterine swelling degree of the model group increased and the uterine coefficient decreased(P<0.05),suggesting that the model was successfully established.Compared with the model group,the uterine swelling degree of each administration group decreased(P<0.05),especially in the high and medium dose groups of Chinese medicine group and the human placental tissue fluid group.Compared with the model group,the expression level of ICAM-1 protein in the uterus tissue of rats in each administration group decreased,and the expression level of caspase-8 protein increased(P<0.05),suggesting that the treatment was effective.There were no significant differences in the expression levels of ICAM-1 and caspase-8 between the blank group and the high dose group of Chinese medicine,the medium dose group of Chinese medicine and the human placental tissue fluid group(P>0.05),suggesting the above three groups had same efficacy.Conclusion The No.2 Gao's Penyan prescription has a antibacterial effect,which can effectively improve the symptoms of the disease,control the development of intrauterine adhesions,and repair the damaged uterine tissue morphology and structure.Its therapeutic mechanism may be related to the down-regulation of ICAM-1 expression and the up-regulation of caspase-8 expression,which can promote the apoptosis of inflammatory cells and enhance the body resistance.
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