Preliminary study on the intestinal protective mechanism of Lactobacillus reuteri on sepsis mouse
Objective To investigate the mechanism of Lactobacillus reuteri in reconstructing the microecological environment of intestinal flora and repairing intestinal mucosa in sepsis mouse.Methods Fifty-four 4-week-old male mice were randomly divided into healthy control group,sepsis group and Lactobacillus re ute ri intervention sepsis group,with 18 mice in each group.The sepsis mouse model was made in the sepsis group and the Lactobacillus reuteri intervention sepsis group.Seven day survival and body activity changes of 10 mice in each of the three groups were measured;the pathological changes of intestinal tissue of mice were observed by HE coloration.Five mice were taken from each of the three groups,and the levels of interleukin-22(IL-22),interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and immunoglobulin A(IgA)of them were determined by enzyme-linked immunosorbent assay(ELISA);the expression of IL-22,IL-1β,IL-6 and TNF-α mRNA in intestinal tissue of mice was detected by fluorescence quantitative PCR.Results The survival of the Lactobacillus reuteri intervention sepsis group was higher than that of the sepsis group(P<0.001).The levels of IL-22,IL-1β,IL-6,TNF-α and their mRNA expression of ileocecal intestinal tissue in the sepsis group were higher than those in the healthy control group(P<0.05);the levels of IL-22,IL-1β,IL-6,TNF-α and their mRNA expression of ileocecal intestinal tissue in the Lactobacillus reuteri intervention sepsis group were lower than those in the sepsis group(P<0.05);the IgA level in the sepsis group was lower than that in the healthy control group(P<0.05);the IgA level in the Lactobacillus reuteri intervention sepsis group was higher than that in the sepsis group,but was lower than that in the healthy control group(P<0.05).Conclusion Lactobacillus reuteri can play an active role in reducing mortality and intestinal barrier protection in sepsis mouse by inhibiting inflammatory response and immune protection regulation.
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