Diagnostic value of Mycobacterium tuberculosis DNA detection in tuberculosis
Objective To study the diagnostic value of Mycobacterium tuberculosis(MTB)DNA detection in tuberculosis.Methods A total of 102 patients with tuberculosis admitted in Nanzheng District People's Hospital from October 2021 to September 2022 were selected as the study group,and 67 patients with other lung diseases during the same period were selected as the control group.Sputum samples of all patients were collected by double-blind experiment;MTB DNA in sputum of all patients was detected by real-time fluorescence quantification polymerase chain reaction(RT-PCR),confirmatory diagnosis was performed by Xpert MTB/RIF detection.The results of bacterial culture were used as the gold standard,the diagnostic efficacy of different detection methods for tuberculosis was compared;the detection of pulmonary tuberculosis and extrapulmonary tuberculosis by different detection methods was compared;the detection of different types of pulmonary tuberculosis by different detection methods was compared.Results A total of 87 cases of tuberculosis were detected by MTB DNA detection,including 3 cases of misdiagnosis and 18 cases of missed diagnosis;a total of 62 cases of tuberculosis were detected by Xpert MTB/RIF detection,including 2 cases of misdiagnosis and 42 cases of missed diagnosis.The sensitivity,accuracy and negative prediction value of MTB DNA detection in the diagnosis of tuberculosis were higher than those of Xpert MTB/RIF detection(P<0.05);there were no significant differences in the specificity and positive prediction value of MTB DNA detection and Xpert MTB/RIF detection in the diagnosis of tuberculosis(P>0.05).The pulmonary tuberculosis detection rate and extrapulmonary tuberculosis detection rate of MTB DNA detection were higher than those of Xpert MTB/RIF detection(P<0.05).The secondary pulmonary tuberculosis detection rate of MTB DNA detection was higher than that of Xpert MTB/RIF detection(P<0.05).Conclusion MTB DNA detection has better diagnostic efficacy for tuberculosis,and its diagnostic value for tuberculosis or extrapulmonary tuberculosis is better than Xpert MTB/RIF detection,which is worthy of promotion.
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