Determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside in different processed products of Saposhnikovia divaricata by HPLC
Objective To establish a method for simultaneous determination of prim-O-glucosylcimifugin and 5-O-methylvisammioside content in Saposhnikovia divaricata,and compare the contents in different processed products.Methods High performance liquid chromatography(HPLC)was used with the Hitachi Lachrom-C18 column(150 mm×4.6 mm,5 μm),methanol-water(40∶60)were used as the mobile phase,the detection wavelength was set at 254 nm,the column temperature was 30 ℃,the flow rate was 1.0 mL/min,and the injection volume was 3 μL.At the same time,the rapid moisture detector was used to determine the moisture content of different processed products.Results There was a good relationship between prim-O-glucosylcimifugin and 5-O-methylvisammioside in the ranges of 14.75-118.00 μg/mL and 24.00-192.00 μg/mL,respectively.The relative standard deviation(RSD)of precision of prim-O-glucosylcimifugin and 5-O-methylvisammioside were 0.68％and 1.13％,the RSD of reproducibility were 0.64％and 1.74％,and the average recoveries were 100.80％and 95.65％,respectively.The content of prim-O-glucosylcimifugin and 5-O-methylvisammioside decreased in different degrees after processing.Although the content of 5-O-methylvisammioside in microwave processed fried Saposhnikovia divaricata was lower than that of traditional processed products,the content of microwave processed products was higher than that of traditional processed products,after processing,the moisture content decreased significantly,and the moisture content of microwave processed products was slightly higher than that of traditional processed products.Conclusion The HPLC method is fast,simple,accurate and reproducible,and can be used for the quality control of different processed products of Saposhnikovia divaricata.The microwave processed products of Saposhnikovia divaricata had higher content of active ingredients and slightly lower moisture content.
万方数据
维普
