Application value of FQ-PCR detection of hepatitis B virus DNA and time-resolved immunofluorescence detection of five items of hepatitis B in the diagnosis of hepatitis B
Objective To investigate the application value of fluorescence quantitative polymerase chain reaction(FQ-PCR)detection of hepatitis B virus(HBV)-DNA and time-resolved immunofluorescence detection of five items of hepatitis B in the diagnosis of hepatitis B.Methods A total of 82 patients with suspected hepatitis B received in our hospital from January 2021 to October 2023 were selected as the research objects,and HBV-DNA was detected by FQ-PCR.At the same time,five items of hepatitis B[hepatitis B virus core antibody(HBcAb),hepatitis B virus e antibody(HBeAb),hepatitis B virus e antigen(HBeAg),hepatitis B virus surface antigen(HBsAg),hepatitis B virus surface antibody(HBsAb)]were detected by time-resolved immunofluorescence,and the test results were analyzed.Results Among 82 patients,the positive rate of HBV-DNA was 70.73%(58/82).Among 82 patients,combination A(HBcAb+HBeAg+HBsAg positive)accounted for 91.46%(75/82),combination B(HBeAb+HBsAg positive)accounted for 63.41%(52/82),combination C(HBcAb+HBeAb+HBsAg positive)accounted for 37.80%(31/82),combination D(HBcAb+HBeAb+HBsAb positive)accounted for 6.10%(5/82),and combination E(HBeAg+HBsAg positive)accounted for 64.63%(53/82).The positive rates of HBV-DNA in combination A,combination B,combination C,combination D and combination E were 53.33%(40/75),7.69%(4/52),29.03%(9/31),40.00%(2/5)and 5.66%(3/53),respectively,the positive rate of HBV-DNA in combination A was higher than that of other combinations,and the difference was statistically significant(P<0.05).The accuracy and sensitivity of FQ-PCR detection of HBV-DNA combined with time-resolved immunofluorescence detection of five items of hepatitis B for the diagnosis of hepatitis B were higher than those of FQ-PCR detection of HBV-DNA and time-resolved immunofluorescence detection of five items of hepatitis B for the diagnosis of hepatitis B alone,and the differences were statistically significant(P<0.05).Conclusion In the diagnosis of hepatitis B,FQ-PCR detection of HBV-DNA and time-resolved immunofluorescence detection of five items of hepatitis B have their own advantages,active combined application will help to further improve the diagnostic efficiency.
fluorescence quantitative polymerase chain reactionhepatitis B virus DNAtime-resolved immunofluorescence assayfive items of hepatitis B
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