FQ-PCR检测乙型肝炎病毒DNA与时间分辨免疫荧光法检测乙肝五项在乙肝诊断中的应用价值
Application value of FQ-PCR detection of hepatitis B virus DNA and time-resolved immunofluorescence detection of five items of hepatitis B in the diagnosis of hepatitis B
柯秋莉 1梁晓芳 1户丹1
作者信息
- 1. 安阳市第五人民医院检验科,河南 安阳,455000
- 折叠
摘要
目的 探讨荧光定量聚合酶链反应(FQ-PCR)检测乙型肝炎病毒(HBV)-DNA与时间分辨免疫荧光法检测乙肝五项在乙肝诊断中的应用价值.方法 选择 2021 年 1月至 2023 年 10 月本院接收的82 例疑似乙肝患者作为研究对象,均通过FQ-PCR实施HBV-DNA检测,同时予以时间分辨免疫荧光法检测乙肝五项[乙肝病毒核心抗体(HBcAb)、乙肝病毒e抗体(HBeAb)、乙肝病毒e抗原(HBeAg)、乙肝表面抗原(HBsAg)、乙肝表面抗体(HBsAb)],并分析检测结果.结果 82例患者中,HBV-DNA阳性率为 70.73%(58/82).82 例患者中,组合A(HBcAb+HBeAg+HBsAg阳性)占比 91.46%(75/82),组合B(HBeAb+HBsAg阳性)占比 63.41%(52/82),组合C(HBcAb+HBeAb+HBsAg阳性)占比 37.80%(31/82),组合D(HBcAb+HBeAb+HBsAb阳性)占比6.10%(5/82),组合E(HBeAg+HBsAg阳性)占比64.63%(53/82).组合A、组合B、组合C、组合D、组合E中HBV-DNA阳性率分别为 53.33%(40/75)、7.69%(4/52)、29.03%(9/31)、40.00%(2/5)、5.66%(3/53),组合A中HBV-DNA阳性率均高于其他各项组合,差异具有统计学意义(P＜0.05).FQ-PCR检测HBV-DNA联合时间分辨免疫荧光法检测乙肝五项诊断乙肝的准确度、灵敏度高于单独FQ-PCR检测HBV-DNA及时间分辨免疫荧光法检测乙肝五项,差异具有统计学意义(P＜0.05).结论 在乙肝诊断中,FQ-PCR检测HBV-DNA与时间分辨免疫荧光法检测乙肝五项各具优势,积极联合应用有助于进一步提升诊断效能.
Abstract
Objective To investigate the application value of fluorescence quantitative polymerase chain reaction(FQ-PCR)detection of hepatitis B virus(HBV)-DNA and time-resolved immunofluorescence detection of five items of hepatitis B in the diagnosis of hepatitis B.Methods A total of 82 patients with suspected hepatitis B received in our hospital from January 2021 to October 2023 were selected as the research objects,and HBV-DNA was detected by FQ-PCR.At the same time,five items of hepatitis B[hepatitis B virus core antibody(HBcAb),hepatitis B virus e antibody(HBeAb),hepatitis B virus e antigen(HBeAg),hepatitis B virus surface antigen(HBsAg),hepatitis B virus surface antibody(HBsAb)]were detected by time-resolved immunofluorescence,and the test results were analyzed.Results Among 82 patients,the positive rate of HBV-DNA was 70.73%(58/82).Among 82 patients,combination A(HBcAb+HBeAg+HBsAg positive)accounted for 91.46%(75/82),combination B(HBeAb+HBsAg positive)accounted for 63.41%(52/82),combination C(HBcAb+HBeAb+HBsAg positive)accounted for 37.80%(31/82),combination D(HBcAb+HBeAb+HBsAb positive)accounted for 6.10%(5/82),and combination E(HBeAg+HBsAg positive)accounted for 64.63%(53/82).The positive rates of HBV-DNA in combination A,combination B,combination C,combination D and combination E were 53.33%(40/75),7.69%(4/52),29.03%(9/31),40.00%(2/5)and 5.66%(3/53),respectively,the positive rate of HBV-DNA in combination A was higher than that of other combinations,and the difference was statistically significant(P<0.05).The accuracy and sensitivity of FQ-PCR detection of HBV-DNA combined with time-resolved immunofluorescence detection of five items of hepatitis B for the diagnosis of hepatitis B were higher than those of FQ-PCR detection of HBV-DNA and time-resolved immunofluorescence detection of five items of hepatitis B for the diagnosis of hepatitis B alone,and the differences were statistically significant(P<0.05).Conclusion In the diagnosis of hepatitis B,FQ-PCR detection of HBV-DNA and time-resolved immunofluorescence detection of five items of hepatitis B have their own advantages,active combined application will help to further improve the diagnostic efficiency.
关键词
荧光定量聚合酶链反应/乙型肝炎病毒DNA/时间分辨免疫荧光法/乙肝五项Key words
fluorescence quantitative polymerase chain reaction/hepatitis B virus DNA/time-resolved immunofluorescence assay/five items of hepatitis B引用本文复制引用
出版年
2024
NETL
NSTL
万方数据