Objective To observe the effect of berberine(BER)on the proliferation,apoptosis and inflammatory factor secretion of human chondrocytes osteoarthritis(HC-OA).Methods HC-OA cells were selected for culture,the blank control group was routinely cultured,and the experimental group was treated with different concentrations of BER on this basis.CCK-8 kit was used to detect the change of cell proliferation ability;real-time quantitative PCR was used to detect the expression of apoptosis genes of BCL-2,BAX and Caspase 3;enzyme linked immunosorbent assay(ELISA)was used to detect the inflammatory factors of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)levels.Results After HC-OA cells were treated with BER for 72 h,compared with the blank control group,different concentrations of BER could significantly promote the proliferation of HC-OA,and the higher the concentration,the more significant the proliferation activity(P<0.05).Compared with the blank control group,BER could significantly down-regulate the expression of apoptosis genes of BAX and Caspase 3,and up-regulate the expression of anti-apoptosis gene of BCL-2(P<0.05).Compared with the blank control group,the levels of IL-1β and TNF-α secreted by cells were significantly up-regulated after LPS induction;compared with LPS group,the levels of IL-1β and TNF-α secreted by cells in the LPS+BER group were significantly down-regulated(P<0.05).Conclusion BER can significantly interfere with the expression of apoptotic genes and anti-apoptotic genes in vitro,promote the proliferation of HC-OA,inhibit its apoptosis and reduce the secretion of inflammatory factors.
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