Effect of berberine on the proliferation,apoptosis and inflammatory factor secretion of human chondrocytes osteoarthritis
Objective To observe the effect of berberine(BER)on the proliferation,apoptosis and inflammatory factor secretion of human chondrocytes osteoarthritis(HC-OA).Methods HC-OA cells were selected for culture,the blank control group was routinely cultured,and the experimental group was treated with different concentrations of BER on this basis.CCK-8 kit was used to detect the change of cell proliferation ability;real-time quantitative PCR was used to detect the expression of apoptosis genes of BCL-2,BAX and Caspase 3;enzyme linked immunosorbent assay(ELISA)was used to detect the inflammatory factors of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)levels.Results After HC-OA cells were treated with BER for 72 h,compared with the blank control group,different concentrations of BER could significantly promote the proliferation of HC-OA,and the higher the concentration,the more significant the proliferation activity(P<0.05).Compared with the blank control group,BER could significantly down-regulate the expression of apoptosis genes of BAX and Caspase 3,and up-regulate the expression of anti-apoptosis gene of BCL-2(P<0.05).Compared with the blank control group,the levels of IL-1β and TNF-α secreted by cells were significantly up-regulated after LPS induction;compared with LPS group,the levels of IL-1β and TNF-α secreted by cells in the LPS+BER group were significantly down-regulated(P<0.05).Conclusion BER can significantly interfere with the expression of apoptotic genes and anti-apoptotic genes in vitro,promote the proliferation of HC-OA,inhibit its apoptosis and reduce the secretion of inflammatory factors.
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