Application value of different instruments in HIV antigen and antibody detection
Objective To compare the application value of two automatic chemiluminescence immunoassay analyzers in the detection of human immunodeficiency virus(HIV)antigen and antibody.Methods A total of 18 745 health managers who underwent physical examination in Jinhu County People's Hospital from January 2022 to December 2023 were selected as the research objects.Roche cobas8000 and Yahuilong iFlash3000 were used to detect HIV antigen and antibody in the collected blood samples,and the positive screening samples were confirmed by immunoblotting.Results The amount of serum required in the Roche cobas8000 was less than that in the Yahuilong iFlash3000,and the detection time was shorter than that in the Yahuilong iFlash3000,and the differences were statistically significant(P＜0.05).The positive detection rate of HIV antigen and antibody in the Roche cobas8000 was 0.41％,which was higher than 0.19％in the Yahuilong iFlash3000,and the difference was statistically significant(P＜0.05).The accuracy of HIV antigen and antibody positive detection in the Roche cobas8000 was 46.75％,which was lower than 97.40％in the Yahuilong iFlash3000,and the difference was statistically significant(P＜0.05).There were 41 false positive samples in the Roche cobas8000 HIV antigen and antibody detection,of which 97.56％(40/41)were distributed in cut off index(COI)1.0-10.0,and 2.44％(1/41)were distributed in COl＞10.0.Conclusion Roche cobas8000 requires less serum to detect HIV antigen and antibody,and the test time is short,but the false positive rate is high.The accuracy of Yahuilong iFlash3000 is higher,and the detection instrument can be selected according to the specific situation.
human immunodeficiency virusRoche cobas8000Yahuilong iFlash3000immunoblotting
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