Expression and clinical significance of miR-6736-3p in gastric adenocarcinoma tissues
Objective To investigate the expression and clinical significance of miR-6736-3p in gastric adenocarcinoma tissues.Methods The gastric adenocarcinoma tissues and adjacent normal tissues of 73 patients were collected,the expression of miR-6736-3p in gastric adenocarcinoma tissues and adjacent normal tissues was detected by qRT-PCR,and the relationship between the expression level of miR-6736-3p and the clinicopathological features and prognosis of gastric adenocarcinoma was analyzed.The growth and proliferation of gastric adenocarcinoma were detected by plate cell clone formation assay and CCK-8 assay.Results The results of qRT-PCR showed that the expression level of miR-6736-3p in the gastric adenocarcinoma tissues was higher than that in the adjacent normal tissues(P<0.05);the expression level of miR-6736-3p in the gastric adenocarcinoma AGS cells was higher than that in the GES-1 cells(P<0.05).The expression level of miR-6736-3p was closely related to tumor size,differentiation degree and prognosis(P<0.05).In vitro cell experiments showed that the number of cell clone formation and optical density(OD)value at 72 h in the miR-6736-3p overexpression group were higher than those in the control group(P<0.05);the number of cell clone formation and OD value at 72 h in the miR-6736-3p decreased expression group were lower than those in the control group(P<0.05).Conclusion miR-6736-3p is highly expressed in gastric adenocarcinoma tissues,which is closely related to tumor size and differentiation degree.It may be an oncogenic factor that can promote the growth and proliferation of gastric adenocarcinoma.
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