Objective To explore the role and mechanism of peptide CRY-14 in myocardial ischemia and hy-poxia.Methods The effects of peptide CRY-14 on the proliferation and oxidative stress of H9C2 myocardial cells after ischemia and hypoxia was analyzed.The effects of peptide CRY-14 on cardiac function(LVEF,LVFS,LVEDD,LVESD)were compared after myocardial infarction in mice by M-type small animal heart ultrasound.HE staining,Tunel staining and Masson staining were used to evaluate the changes of cardiac histology and apoptosis after the intervention of CRY-14.In addition,Western Blot assay was used to investigate the mechanism of myocardial protection of peptide CRY-14.Results At the cellular level,CRY-14 alleviated the inhibitory effect of ischemia and hypoxia on the proliferation of H9C2 cardiomyocytes,and reduced the oxidative stress response caused by ischemia and hypoxia.In vivo,CRY-14 alle-viated the changes of cardiac function after myocardial infarction,increase LVEF and LVFS,and decrease LVEDD and LVESD.CRY-14 improved significantly myocardial injury,reduced myocardial cell apoptosis and myocardial fibrosis in mice after myocardial infarction.In addition,Western blot results suggested that CRY-14 can downregulate the expression of apoptosis related protein Caspase 3 and PARP in H9C2 cardiomyocytes after ischemia and hypoxia,while CRY-14 up-regulated the expression of HSP70 in H9C2 cardiomyocytes after ischemia and hypoxia.Conclusions Peptide CRY-14 plays a myocardial protective role by improving oxidative stress and apoptosis levels in ischemic heart disease,and its mechanism may be related to the regulation of HSP70.
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