摘要
再生体系遗传转化是现代生物技术的一个重要手段,该技术转化基因稳定、成本低效益高.但基因依赖型和顽抗性植物辣椒对农杆菌不敏感,导致辣椒再生体系遗传转化效率低,且存在基因转移整合或目的基因脱靶等问题.为此,综述了农杆菌介导辣椒遗传转化的基因型、受体、外源添加物、发育调节因子、编辑器、接种物、预培养、侵染时间、共培养以及褐化等影响因素,以期降低辣椒遗传转化风险,提高辣椒再生体系遗传转化效率.
Abstract
Genetic transformation is an important method in the modern biotechnology,which can stably transfer genes with low cost and high benefits.However,the genotype-dependent phenomenon and recalcitrant pepper germplasm,together with the problems of transgenic integration and off-target,result in low efficiency in genetic transformation in pepper.The effects of genotypes,receptors,exogenous additives,developmental regulators,editors,inoculants,pre-culture,infection time,co-culture,and browning on pepper mediated genetic transformation by Agrobacterium were reviewed in this paper with the aims of decreasing the risk and increasing the efficiency of pepper genetic transformation.
国家科技期刊平台
NSTL
维普
万方数据