首页|农杆菌介导辣椒遗传转化的影响因素

农杆菌介导辣椒遗传转化的影响因素

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再生体系遗传转化是现代生物技术的一个重要手段,该技术转化基因稳定、成本低效益高.但基因依赖型和顽抗性植物辣椒对农杆菌不敏感,导致辣椒再生体系遗传转化效率低,且存在基因转移整合或目的基因脱靶等问题.为此,综述了农杆菌介导辣椒遗传转化的基因型、受体、外源添加物、发育调节因子、编辑器、接种物、预培养、侵染时间、共培养以及褐化等影响因素,以期降低辣椒遗传转化风险,提高辣椒再生体系遗传转化效率.
Analysis on the Factors Affecting Agrobacterium-mediated Genetic Transformation in Capsicum annuum L.
Genetic transformation is an important method in the modern biotechnology,which can stably transfer genes with low cost and high benefits.However,the genotype-dependent phenomenon and recalcitrant pepper germplasm,together with the problems of transgenic integration and off-target,result in low efficiency in genetic transformation in pepper.The effects of genotypes,receptors,exogenous additives,developmental regulators,editors,inoculants,pre-culture,infection time,co-culture,and browning on pepper mediated genetic transformation by Agrobacterium were reviewed in this paper with the aims of decreasing the risk and increasing the efficiency of pepper genetic transformation.

pepperregeneration systemgenetic transformationAgrobacterium

许晴、张宏冠、赵红杰、龙醌、吴明秋、刘震桦、胡博文

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湖南农业大学园艺学院,长沙 410128

辣椒 再生体系 遗传转化 农杆菌

湖南省重点研发计划

2023NK2006

2024

辣椒杂志
辣椒新品种技术研究推广中心

辣椒杂志

影响因子:0.205
ISSN:1672-4542
年,卷(期):2024.22(1)
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