Analysis on the Factors Affecting Agrobacterium-mediated Genetic Transformation in Capsicum annuum L.
Genetic transformation is an important method in the modern biotechnology,which can stably transfer genes with low cost and high benefits.However,the genotype-dependent phenomenon and recalcitrant pepper germplasm,together with the problems of transgenic integration and off-target,result in low efficiency in genetic transformation in pepper.The effects of genotypes,receptors,exogenous additives,developmental regulators,editors,inoculants,pre-culture,infection time,co-culture,and browning on pepper mediated genetic transformation by Agrobacterium were reviewed in this paper with the aims of decreasing the risk and increasing the efficiency of pepper genetic transformation.