Optimization of the Genetic Transformation System for the Ecotype Cucumber'CU2'in South China
Cucumber is a vegetable crop with significant economic and biological values.The widespread application of genome editing technology has promoted the rapid development of cucumber breeding and improvement,as well as researches on functional genomics.At present,the South China ecotype cucumber germplasm still lack a universally applicable Agrobacterium-mediated genetic transformation system,which limits the efficient application of genome editing technology.This study used the South China ecotype cucumber germplasm'CU2'as the material and the creation of CsMATL gene-edited transgenic plants as an example to optimize several factors in the genetic transformation system,achieving the highest genetic transformation rate of explant differentiation buds.The results showed that the operations of sowing the seeds on the SGM medium with 2 mg/L 6-BA and 1 mg/L ABA,co-culturing the explants at a concentration of 200 μmol/L AS,and removing bacteria using two 200 mg/L TMT washes and three sterile water washes on a shaker yielded the positive results.Then,the differentiation culture was carried out in a differentiation medium containing 100 mg/L Kan,2 mg/L 6-BA,and 1 mg/L ABA.The genetic transformation rate of'CU2'can be increased to 3.19%by using the optimized method.This study laid a theoretical foundation for optimizing genetic transformation system and manipulating functional genes in South China ecotype cucumber.
NETL
NSTL
万方数据
