Objective:To summarize the clinical application of metagenomic next-generation sequencing(mNGS)in the diagnosis of rabies.Methods:The diagnosis and treatmentdataof1caseof rabies in our hospital and 3 cases of litera-ture searchwere retrospectively.Results:4 patients were tested positive for rabies virus by mNGS.3 patients were tested positive by PCR at the same time,providing a basis for clinical diagnosis of rabies.Conclusion:Itprovedaclinicaldiag-nosticbasisofrabiesdetected samplesofsalivary and cerebrospinal fluid by mNGS.