Expression of S100A8/S100A9-related Pathway Proteins in Plasma Circulation in Patients with Traumatic Shock and SIRS
Objective:To investigate the expression of S100A8/S100A9 related pathways in patients with traumatic shock and SIRS.Methods:90 patients diagnosed with traumatic shock in our hospital were included in the prospective study,including 43 patients with SIRS,and 45 healthy subjects as the control group.The plasma circulating S100A8/A9 and inflammatory factors in each group were detected.A rat model of traumatic shock with SIRS was established,and S100A8/A9 levels were detected by qRT-PCR and Western Blot.Results:The plasma levels of S100A8/A9 andIL-6 and TNF-β in the patients with traumatic shock and SIRS were significantly higher than those in the patients without SIRS and control group(P<0.05).Plasma S100A8/A9 protein,IL-6 and TNF-β were also significantly increased in model group,while S100A8-mRNA,S100A9-mRNA and S100A8/A9 protein levels in lung and liver tissues were also signifi-cantly higher than those in control group(P<0.05).Correlation analysis showed that the levels of circulating plasma S100A8/A9 were positively correlated with inflammatory cytokines IL-6 and TNF-β(r=0.874,0.748,P=0.000).Conclu-sion:As a new inflammatory mediator,S100A8/A9 participated in the occurrence and development of SIRS in patients with traumatic shock.Its mechanism may have synergistic effect with pro-inflammatory factor TNf-alpha and IL-6,which jointly exacerbated the organ injury of traumatic shock accompanied by SIRS.
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