Performance Analysis of Immunohistochemical Staining for Breast Cancer using Different Ki-67 Clones
Objective:To investigate the expression percentage of different Ki-67 clones in breast invasive ductal carcinoma tissue.Methods:Data from 45 patients with invasive ductal carcinoma of the breast admitted to the Depart-ment of Pathology at Sun Yat-sen Memorial Hospital of Sun Yat sen University from 2022 to 2023 were collected.Immu-nohistochemical methods were used to explore the expression of Ki-67 in these patients.Through comparative analysis,the difference of increment index percentage of different clone Ki-67 in breast cancer was compared.Results:In this analysis of 45 cases,statistically significant differences werenoticed between the median proliferation index of Ki-67 in MIB1,C3G4,30-9,and UMAB107(the median proliferation index,10.97,4.74,16.09,and 9.87,respectively).Con-clusion:The selection of antibody clone has great influence on Ki-67 immunohistochemical staining results in breast can-cer,The percentage of positive cells of Ki-67 antibodies with different clones in breast cancer was significantly different,Ki-67(30-9)and(MIB1)have high and stable expression in breast cancer.It is recommended to use 30-9 and MIB1 in breast cancer Ki-67 immunohistochemical detection process.
万方数据
维普
