In order to clarify the species and biological characteristics of pathogenic bacteria of common leaf diseases at maize mature-plant stage in Yingkou City,three main pathogenic bacteria of leaf diseases were identified by morphology and mo-lecular biology.The growth rate and stress resistance of three pathogenic bacteria were determined by cross method.The results of morphological identification showed that the three pathogenic fungi Cl-1,Ch-1 and Aa-1 were Curvularia lunata,Bipolaris may-dis and Alternaria alternata,respectively.Combined with phylogenetic analysis,Cl-1 was Curvularia lunata,Ch-1 was Bipolaris maydis and Aa-1 was Alternaria alternata.Among them,leaf spot of maize is a new disease of leaf disease at mature-plant stage in Yingkou City.The results of stress resistance determination of pathogenic bacteria showed that among the three stress factors,Curvularia Lunata,Bipolaris maydis and Alternaria alternata were more sensitive to 1 mol/L NaCl,and their growth was signifi-cantly inhibited.They were sensitive to 0.005%SDS and 10 mmol/L H2O2,but their growth was not significantly inhibited com-pared with the control.
关键词
玉米叶部病害/形态学鉴定/分子生物学鉴定/抗逆性测定
Key words
maize leaf disease/morphological identification/molecular biological identification/determination of stress resistance
维普
万方数据