Using sunflower seed meal as raw material,the effects of pH value,extraction time,solid-liquid ratio and extraction temperature on ultrasonic assisted protein extraction were investigated by single factor test and orthogonal test,so as to optimize the extraction process and improve the extraction rate of protein.The results showed that the influence of each single factor on ultrasonic assisted protein extraction was the ratio of solid to liq-uid,pH value,extraction temperature and extraction time in descending order.The optimal process conditions were pH value of 8.5,extraction time of 85 min,solid-liquid ratio of 1∶25(g/mL),extraction temperature of 50 ℃,and the extraction rate of protein was 72.58%.This study provided technical reference for the efficient ex-traction of protein from sunflower seed meal.
关键词
超声波/碱溶酸沉法/葵花籽饼粕/蛋白质
Key words
ultrasonic/alkali solution and acid precipitation/sunflower seed meal/protein