Objective: The purpose of this research is to induce the callus of Epimedium through tissue culture technology. This study lays the foundation for the subsequent extraction and secondary metabolites determination. Methods: The suitable conditions of Epimedium callus induction were studied in this paper, based on the experimental material of the underground bud and young leaf. Result: Callus induction rate of underground bud is higher than young leaf. Induction rate is highest on MS containing 20g/L sucrose,6g/L agar, 1.0mg/L 6-BA and 1.0mg/L NAA. Conclusion: The underground bud is the best experimental material in the callus induction from epimedium.
NETL
NSTL
万方数据
维普
