A Study on the Changes of Lung Microbiota in Mice with Influenza Virus Pneumonia before and after Intervention with Maxing Shigan Decoction(麻杏石甘汤)
Objective To investigate the changes in lung microbiota in mice before and after infection with influenza virus pneumonia,and the effect of intervention with Maxing Shigan Decoction(麻杏石 甘汤)on lung microbiota in mice.Methods A mouse model of influenza virus pneumonia was established by nasal drip modeling.After intervention with Maxing Shigan Decoction or physiological saline for different times,mouse lung tissue was extracted for 16S rDNA sequencing data processing and personality analysis,to explore the effect of Maxing Shigan Decoction on the lung microbiota of influenza virus pneumonia mice.Results The 16S rDNA sequencing results showed that the relative abundance of Firmicutes and Bacteroidetes decreased in the model group,while the relative abundance of Proteobacteria,Pantobacteria,and Shigella increased.The Maxing Shigan Decoction group and the oseltamivir group can reduce the relative abundance of Proteobacteria and Shigella,and increase the relative abundance of Firmicutes.Alpha diversity analysis showed that after 7 days of administration,the richness and diversity of lung microbiota in the Maxing Shigan Decoction group and the oseltamivir group of mice increased compared to the model group(P＜0.05);The Beta diversity analysis results showed that after 7 days of administration,the distance between sample points in each group except for the model group was less than 3 days after administration,and there were differences in the composition of lung microbiota between the groups.Conclusion After intervening with influenza virus pneumonia in mice,Maxing Shigan Decoction promotes the growth of beneficial bacteria in the lung microbiota of mice and reduces the degree of lung tissue damage.
万方数据
维普
