Study on the Mechanism of Action of Bixie Shenshi Decoction(萆薢渗湿汤)on Acute Eczema Mouse Model
Objective To explore the therapeutic effect and mechanism of Bixie Shenshi Decoction(萆薢渗湿汤)on acute eczema mouse model.Methods Sixty SPF KM male mice were randomly divided into 6 groups after adaptive feeding for 7 days,with 10 mice in each group.The model was established by applying 2,4-dinitrochlorophenylpropanone solution.The rats were divided into normal control group,model control group,prednisone positive drug group[drug dose of 10 mg/(kg·d),equivalent to 10 times the clinical dose],Bixie Shenshi Decoction low,medium and high dose group(drug doses were 0.70,1.40,2.80 g/kg,equivalent to 0.5,1,2 times the clinical equivalent dose).Twenty-four hours after the model was stimulated,the mice in the prednisone positive drug group and the high,medium and low dose groups of Bixie Shenshi Decoction were given 0.1 mL/10 g of the corresponding drugs by gavage once a day for 7 days.The normal control group and the model control group were given purified water,and the mice were fed normally during the administration.The general condition of the mice in each group,the improvement of ear skin lesions,and the spleen index were observed.The tumor necrosis factor-α(TNF-α),interleukin-2(IL-2),and interleukin-4(IL-4)in each group were detected by ELISA.Results Compared with the normal control group,the weight and thickness of the right ear of the mice in the model control group increased significantly(P＜0.01),and the thickness and weight of the left ear did not change significantly.Compared with the model control group,the weight of the right ear in the prednisone positive drug group and the high and middle dose of Bixie Shenshi Decoction groups were significantly reduced(P＜0.01),and the thickness of the right ear in the prednisone positive drug group and the high dose of Bixie Shenshi Decoction group were significantly reduced(P＜0.01).There was no significant change in the weight and thickness of the left ear in the prednisone positive drug group and each dose of Bixie ShenShi Dection group.Compared with the normal control group,the spleen index of the model control group was significantly increased(P＜0.01).Compared with the model control group,the spleen index of the prednisone positive drug group and the high dose of Bixie ShenShi Dection group were significantly decreased(P＜0.05,P＜0.01).Compared with the normal control group,the serum levels of IL-2,IL-4 and TNF-α in the model control group were significantly increased(P＜0.01).Compared with the model control group,the serum levels of IL-2,IL-4 and TNF-α in the prednisone positive drug group and each dose group of Bixie Shenshi Decoction were significantly decreased(P＜0.01).Conclusion Bixie Shenshi Decoction can treat skin inflammation in acute eczema model of mice,and its mechanism may be related to the reduction of inflammatory factors L-2,IL-4 and TNF-α in serum of mice.
万方数据
维普
