Rapid Determination of Amanita Peptide Toxins in Mushrooms by Ultra Performance Liquid Chromatography-Q Exactive orbitrap high-resolution mass spectrometry
The ultra performance liquid chromatography-Q Exactive orbitrap high-resolution mass spectrometry method was established for the rapid determination of 3 kinds of amanita peptide toxins in mushrooms.The sample was abstracted with the standard mixed solution of formic acid-methanol-water(0.5∶10∶89.5,v/v/v)after ho-mogenized.The amanita peptide toxins were purified with BEH shield RP18 chromatographic analysis column elu-tion(150 mm ×2.1 mm,1.7 μm),Gradient elution with a mobile phase of 0.03％ammonia.tSIM positive ion mode scanning was used for detection under HESI,and quantitative analysis was carried out by external standard method.The linearities of 3 kinds of amanita peptide toxins in mushrooms were good in range of 0.05 ng/mL to 5 mg/kg,correlation coefficients r were all above 0.995,and the detection limit of α-amanitracin(α-AMA)and α-amanitracin(γ-AMA)was 0.01 mg/kg,and the limit of quantitation was 0.02 mg/kg.The values of β-AMA were 0.02 mg/kg and 0.05 mg/kg respectively.The average recovery was in the range of 72.5％～91.9％at the three added levels,and the relative standard deviation(RSD,n=6)was 2.8％～7.4％.This method is simple and efficient.It can be applied to rapid detection of 3 kinds of amanita peptide toxins in mushrooms.
amanitinsmushroomsUltra Performance Liquid Chromatography-Q Exactive orbitrap high-resolu-tion mass spectrometry
万方数据
维普
