首页|高效液相色谱法和气相色谱法分析肟菌酯原药的方法比较

高效液相色谱法和气相色谱法分析肟菌酯原药的方法比较

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建立高效液相色谱法和气相色谱法,来测定肟菌酯原药有效成分的定量分析方法比较.用Agilent C18 柱,采用甲醇和水为流动相,通过二极管阵列检测器波长为 250 nm;AE.SE-30 毛细管柱,以邻苯二甲酸二环己酯为内标物,FID检测器分别对肟菌酯有效成分进行定量分析.高效液相色谱法和气相色谱法的线性相关系数分别为 0.999 9、0.999 5,标准偏差分别为 0.21、0.19,变异系数分别为 0.21%、0.19%,平均回收率分别为 99.74%和 99.67%.通过比较,两种方法都能很好地用于肟菌酯原药的定量分析.
Comparison of methods for original drug of trifloxystrobin by HPLC and GC
Comparison of methods for quantitative analysis of the active ingredients of trifloxystrobin was determined by HPLC and gas chromatography.By a diode array detector wavelength at 250nm and FID detector,the active in-gredient of trifloxystrobin was analyzed quantitatively With a Agilent C18 column and a AE.SE-30 Capillary column,using methanol and water as the mobile phase and dicyclohexyl phthalat as the internal standard.The results showed that the linear correlation coefficients of HPLC and gas chromatography were 0.999 9 and 0.999 5,the standard devia-tions were 0.21 and 0.19,the coefficient of variation was 0.21%and 0.19%,and the average recovery was 99.74%and 99.67%respectively.By comparison,the both methods are good for quantitative analysis of trifloxystrobin.

HPLCGCtrifloxystrobin

陈颖、刘小锋、陈一萍、廖丽萍、傅洪涛

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湖南化工研究院有限公司 国家农药创制工程技术研究中心,湖南 长沙 410014

湖南化肥农药质量监督检验授权站,湖南 长沙 410014

高效液相色谱 气相色谱 肟菌酯

湖南省科技创新计划重点项目

2022NK2053

2024

粮食科技与经济
湖南省粮食经济科技学会 中诸粮管理总公司湖南分公司

粮食科技与经济

影响因子:0.505
ISSN:1007-1458
年,卷(期):2024.49(1)
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