高效液相色谱法和气相色谱法分析肟菌酯原药的方法比较
Comparison of methods for original drug of trifloxystrobin by HPLC and GC
陈颖 1刘小锋 1陈一萍 1廖丽萍 1傅洪涛1
作者信息
- 1. 湖南化工研究院有限公司 国家农药创制工程技术研究中心,湖南 长沙 410014;湖南化肥农药质量监督检验授权站,湖南 长沙 410014
- 折叠
摘要
建立高效液相色谱法和气相色谱法,来测定肟菌酯原药有效成分的定量分析方法比较.用Agilent C18 柱,采用甲醇和水为流动相,通过二极管阵列检测器波长为 250 nm;AE.SE-30 毛细管柱,以邻苯二甲酸二环己酯为内标物,FID检测器分别对肟菌酯有效成分进行定量分析.高效液相色谱法和气相色谱法的线性相关系数分别为 0.999 9、0.999 5,标准偏差分别为 0.21、0.19,变异系数分别为 0.21%、0.19%,平均回收率分别为 99.74%和 99.67%.通过比较,两种方法都能很好地用于肟菌酯原药的定量分析.
Abstract
Comparison of methods for quantitative analysis of the active ingredients of trifloxystrobin was determined by HPLC and gas chromatography.By a diode array detector wavelength at 250nm and FID detector,the active in-gredient of trifloxystrobin was analyzed quantitatively With a Agilent C18 column and a AE.SE-30 Capillary column,using methanol and water as the mobile phase and dicyclohexyl phthalat as the internal standard.The results showed that the linear correlation coefficients of HPLC and gas chromatography were 0.999 9 and 0.999 5,the standard devia-tions were 0.21 and 0.19,the coefficient of variation was 0.21%and 0.19%,and the average recovery was 99.74%and 99.67%respectively.By comparison,the both methods are good for quantitative analysis of trifloxystrobin.
关键词
高效液相色谱/气相色谱/肟菌酯Key words
HPLC/GC/trifloxystrobin引用本文复制引用
基金项目
湖南省科技创新计划重点项目(2022NK2053)
出版年
2024
NETL
NSTL
万方数据