首页|响应面优化复合酶法提取红薯皮膳食纤维

响应面优化复合酶法提取红薯皮膳食纤维

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为了消除红薯初加工副产物所带来的环境污染问题,提高红薯产品附加值,以红薯皮为原料,采用复合酶法提取其中的膳食纤维.通过单因素实验探究了复合酶的添加量、酶解时间、酶解pH、酶解温度和料液比对红薯皮膳食纤维得率的影响,并采用Box-Behnken试验优化提取参数.结果表明:红薯皮膳食纤维的最佳提取工艺参数为料液比 1∶25(g/mL)、复合酶(纤维素酶∶淀粉酶∶果胶酶质量比=1∶1∶1)添加量 0.5%、酶解时间 1.8 h、酶解pH 5.5、酶解温度 48℃,该工艺条件下红薯皮可溶性膳食纤维得率为23.64%,不溶性膳食纤维得率为 39.88%.
Optimization of response surface for extracting dietary fiber from sweet potato peels using complex enzymes
In order to eliminate the environmental pollution caused by the initial processing by-products of sweet potatoes and improve the added value of sweet potato products,this paper took sweet potato peels as raw materials and explored the effects of compound enzyme addition,enzymatic hydrolysis time,enzymatic hydrolysis pH,enzymatic hydrolysis temperature,and liquid-to-solid ratio on dietary fiber yield through single factor experiments.The response surface method was used to optimize the process parameters.The results showed that the optimal process parameters were a liquid-to-solid ratio of 1:25(g/mL),a compound enzyme addition rate of 0.5%(fiber enzyme:amylase:pectinase=1:1:1),an enzymatic hydrolysis time of 1.8 hours,an enzymatic hydrolysis pH of 5.5,and an enzymatic hydrolysis temperature of 48℃.Under these op-timal process conditions,the yield of soluble dietary fiber from sweet potato peels was 23.64%,and the yield of insoluble dietary fiber was 39.88%.

sweet potato peelsresponse surfacecompound enzyme methoddietary fiber

林良美、肖少香、张丽红

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漳州职业技术学院,福建 漳州 363000

湖南科技职业学院,湖南 长沙 410004

红薯皮 响应面 复合酶法 膳食纤维

漳州职业技术学院校级课题

ZZY2021B141

2024

粮食科技与经济
湖南省粮食经济科技学会 中诸粮管理总公司湖南分公司

粮食科技与经济

影响因子:0.505
ISSN:1007-1458
年,卷(期):2024.49(4)