植物乳植杆菌BXM2产γ-氨基丁酸的发酵条件优化及特性研究
Optimization of fermentation conditions and Characteristics of γ-Aminobutyric Acid Produced by Lactiplantibacillus plantarum BXM2
朱永乐 1宋贵梅 1乔荣更 1湛剑龙 1赵大洲 1吴琼琳1
作者信息
- 1. 厦门元之道生物科技有限公司,福建 厦门 361100
- 折叠
摘要
为了得到较高产量的γ-氨基丁酸(Gamma-Aminobutyric Acid,GABA)发酵工艺,采用单因素实验和响应面优化法对植物乳植杆菌BXM2 产GABA的发酵条件进行优化,并分析菌株BXM2 的耐酸、耐胆盐能力.实验结果表明,产GABA最优发酵条件为菌种接种量 3.7%、发酵温度 37℃、发酵时间 32.2 h,在该发酵条件下,菌株BXM2 产GABA含量高达 479.23 μg·g-1.耐酸、耐胆盐实验结果表明,在不同pH值和含有不同浓度胆盐的MRS培养基中培养 2 h,其活菌数均超过 1×105 CFU·mL-1.菌株BXM2 具有较强的耐胆盐、耐酸能力,在优化发酵条件下可高产GABA,对开发GABA功能性食品具有重要意义.
Abstract
In order to obtain a higher yield of gamma-aminobutyric acid(GABA)fermentation process,single factor experiments and response surface optimization methods were used to optimize the fermentation conditions for GABA production by Lactiplantibacillus plantarum BXM2,and the acid and bile salt resistance of strain BXM2 were analyzed.The experimental results showed that the optimal fermentation conditions for producing GABA were as follows:inoculum volume of 3.7%,fermentation temperature of 37℃,and fermentation time of 32.2 hours.Under these fermentation conditions,the GABA content produced by strain BXM2 reached as high as 479.23 μg·g-1.The results of acid and bile salt resistance experiments showed that when cultured in MRS medium with different pH values and concentrations of bile salt for 2 hours,the number of viable bacteria exceeded 1×105 CFU·mL-1.Strain BXM2 has strong bile salt and acid resistance,and can produce high levels of GABA under optimized fermentation conditions,which is of great significance for the development of GABA functional foods.
关键词
γ-氨基丁酸/植物乳植杆菌BXM2/高效液相色谱仪/发酵优化Key words
gamma-aminobutyric acid/Lactiplantibacillus plantarum BXM2/high performance liquid chromatograph/fermentation optimization引用本文复制引用
基金项目
福建省农产品(食品)加工重点实验室开放课题(NJG2022006)
出版年
2024
NETL
NSTL
万方数据