Optimization of fermentation conditions and Characteristics of γ-Aminobutyric Acid Produced by Lactiplantibacillus plantarum BXM2
In order to obtain a higher yield of gamma-aminobutyric acid(GABA)fermentation process,single factor experiments and response surface optimization methods were used to optimize the fermentation conditions for GABA production by Lactiplantibacillus plantarum BXM2,and the acid and bile salt resistance of strain BXM2 were analyzed.The experimental results showed that the optimal fermentation conditions for producing GABA were as follows:inoculum volume of 3.7%,fermentation temperature of 37℃,and fermentation time of 32.2 hours.Under these fermentation conditions,the GABA content produced by strain BXM2 reached as high as 479.23 μg·g-1.The results of acid and bile salt resistance experiments showed that when cultured in MRS medium with different pH values and concentrations of bile salt for 2 hours,the number of viable bacteria exceeded 1×105 CFU·mL-1.Strain BXM2 has strong bile salt and acid resistance,and can produce high levels of GABA under optimized fermentation conditions,which is of great significance for the development of GABA functional foods.
gamma-aminobutyric acidLactiplantibacillus plantarum BXM2high performance liquid chromatographfermentation optimization
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