山楂多糖酶解法和水提法的工艺优化和比较
Process Optimization and Comparison of Enzymatic Hydrolysis and Water Extraction of Hawthorn Polysaccharide
刘京 1李荣乔 1张胜楠 1李成 1马军1
作者信息
- 1. 石家庄以岭药业股份有限公司,河北 石家庄 050000
- 折叠
摘要
研究酶解法和水提法对山楂多糖提取率的影响,为进一步开发利用山楂多糖提供技术依据.分别对酶解法和水提法进行优化,选择最佳条件提取山楂多糖.结果表明,酶解法提取山楂多糖的最适条件为酶用量 3‰、酶解温度 50℃、酶解时间 1.0 h、酶解pH=8、料液比 1∶10,多糖提取率为 11.72%;水提法提取山楂多糖的最适条件为提取次数 3 次、提取时间 120 min、料液比 1∶10,多糖提取率为 9.47%.酶解法提取率高,优于水提法.结论:山楂中多糖含量较高,通过酶解法可进行高效提取,方法简便,实用性强.
Abstract
The effects of enzymatic hydrolysis and water extraction on the extraction rate of hawthorn polysaccharide were studied to provide technical basis for further development and utilization of hawthorn polysaccharide.The enzymatic method and water extraction method were optimized to select the best conditions for extraction of hawthorn polysaccharide.The results showed that the optimum conditions of extracting hawthorn polysaccharide by enzymatic hydrolysis were as follows:enzyme dosage 3‰,enzymatic hydrolysis temperature 50℃,enzymatic hydrolysis time 1.0 h,enzymatic hydrolysis pH=8,ratio of material to liquid 1 ∶ 10.The extraction rate of polysaccharide was 11.72%.The optimum extraction conditions of hawthorn polysaccharide by water extraction were as follows:extraction 3 times,extraction 120 minutes,ratio of material to liquid 1 ∶ 10.The extraction rate of polysaccharide was 9.47%.Enzymatic hydrolysis is better than water extraction.Conclusion:the content of polysaccharide in hawthorn is high,which can be extracted by enzymatic hydrolysis,and the method is simple and practical.
关键词
山楂/多糖/酶解/水提/提取率Key words
hawthorn/polysaccharides/enzymatic hydrolysis/water extraction/extraction rate引用本文复制引用
基金项目
河北省重点研发计划项目(20327120D)
出版年
2024
NETL
NSTL
万方数据