Process Optimization and Comparison of Enzymatic Hydrolysis and Water Extraction of Hawthorn Polysaccharide
The effects of enzymatic hydrolysis and water extraction on the extraction rate of hawthorn polysaccharide were studied to provide technical basis for further development and utilization of hawthorn polysaccharide.The enzymatic method and water extraction method were optimized to select the best conditions for extraction of hawthorn polysaccharide.The results showed that the optimum conditions of extracting hawthorn polysaccharide by enzymatic hydrolysis were as follows:enzyme dosage 3‰,enzymatic hydrolysis temperature 50℃,enzymatic hydrolysis time 1.0 h,enzymatic hydrolysis pH=8,ratio of material to liquid 1 ∶ 10.The extraction rate of polysaccharide was 11.72%.The optimum extraction conditions of hawthorn polysaccharide by water extraction were as follows:extraction 3 times,extraction 120 minutes,ratio of material to liquid 1 ∶ 10.The extraction rate of polysaccharide was 9.47%.Enzymatic hydrolysis is better than water extraction.Conclusion:the content of polysaccharide in hawthorn is high,which can be extracted by enzymatic hydrolysis,and the method is simple and practical.
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